LOCUS       Exported               10900 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJG091, complete sequence.
ACCESSION   KX499662
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10900)
  AUTHORS   Goodson J, Klupt S, Zhang C, Straight P, Winkler W.
  TITLE     A Broadly Conserved Antiterminator Protein Controls a Regulon of 
            Bacillus amyloliquefaciens Antibiotic Gene Clusters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10900)
  AUTHORS   Goodson JR, Klupt SL.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2016) Cell Biology and Molecular Genetics, 
            University of Maryland, 3112 Bioscience Research Building, College 
            Park, MD 20742, USA
REFERENCE   3  (bases 1 to 10900)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10900
                     /organism="Cloning vector pJG091"
                     /mol_type="other DNA"
                     /country="USA"
                     /note="for ectopic integration into the Bacillus 
                     amyloliquefaciens chromosome."
                     /db_xref="taxon:1930834"
     regulatory      3..107
                     /regulatory_class="promoter"
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        3..107
                     /gene="bla"
                     /label=AmpR promoter
     CDS             108..968
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="ampR; gene=bla - note=ampicillin resistance - 
                     codon_start=1 - transl_table=11 - product=beta-lactamase - 
                     protein_id=AAB40303.1 - db_xref=GI:1185570 - 
                     translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY - 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAG"
                     /protein_id="APU90165.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            108..968
                     /gene="bla"
                     /label=bla
     rep_origin      1139..1727
                     /note="ori; high-copy-number ColE1/pMB1/pBR322/pUC origin 
                     of replication"
     CDS             complement(1974..2756)
                     /codon_start=1
                     /gene="spc"
                     /product="spectinomycin adenyltransferase"
                     /label=spc
                     /note="spectinomycin adenyltransferase; gene=spc - 
                     note=spectinomycin resistance - codon_start=1 - 
                     transl_table=11 - product=spectinomycin adenyltransferase -
                     protein_id=AAB40302.1 - db_xref=GI:1185569 - 
                     translation=MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI - 
                     NSDVDVLVVVNHSLPQLTRKK"
                     /protein_id="APU90166.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRIN
                     SDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYPPK
                     REFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLTDIR
                     RAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEHVTLL
                     DIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(1974..2756)
                     /gene="spc"
                     /label=spc
     misc_feature    3865..4865
                     /pseudo
                     /note="amyE; amyE back; amyE homology for integration into 
                     B. amyloliquefaciens"
     CDS             complement(5236..5886)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetylase"
                     /label=cat
                     /note="chloramphenicol acetylase; gene=cat - 
                     note=chloramphenicol resistance - codon_start=1 - 
                     transl_table=11 - product=chloramphenicol acetylase - 
                     protein_id=AAB40300.1 - db_xref=GI:1185567 - 
                     translation=MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQ - 
                     EGYKFYPAFIFLVTRVINSNTAFRT"
                     /protein_id="APU90167.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     gene            complement(5236..5886)
                     /gene="cat"
                     /label=cat
     regulatory      6196..6337
                     /regulatory_class="promoter"
                     /note="PdfnA"
     5UTR           6345..6745
                     /note="dfnA leader region; dfnA leader region"
     regulatory      6746..6792
                     /regulatory_class="terminator"
                     /note="T7 terminator; transcription terminator for 
                     bacteriophage T7 RNA polymerase"
     terminator      6746..6792
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     regulatory      6794..6839
                     /regulatory_class="terminator"
                     /note="lambda tR2 terminator"
     regulatory      6840..6885
                     /regulatory_class="terminator"
                     /note="rrnB T1 terminator; transcription terminator T1 from
                     the E. coli rrnB gene"
     terminator      6840..6885
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     regulatory      6897..6914
                     /regulatory_class="ribosome_binding_site"
                     /note="Winkler Std. RBS"
     CDS             6915..7631
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="YFP"
                     /protein_id="APU90168.1"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     regulatory      7638..7671
                     /regulatory_class="terminator"
                     /note="YFP_terminator"
     misc_feature    7679..7697
                     /label=amyE-front
                     /note="amyE-front"
     CDS             complement(7745..8275)
                     /codon_start=1
                     /gene="loaP"
                     /product="LoaP"
                     /label=loaP
                     /note="LoaP; LoaP NusG-like antiterminator protein"
                     /protein_id="APU90169.1"
                     /translation="MKWYALFVESGKEETVQKFLRLQFDEQALYSIIPKKKVTERKAGI
                     KYEALKKMFPGYVLFKTKMTERTFHKIKELPISCRIVNNGAYYSKERKTYFTTIKDEEI
                     LPIIRLIGEGDTVDYSKVYIENSKVTVASGPLKGMEGIIKKIDKRKRRAKICLSFMGLD
                     KMVNVGIEVLSKP"
     gene            complement(7745..8275)
                     /gene="loaP"
                     /label=loaP
     CDS             complement(8304..8363)
                     /codon_start=1
                     /gene="mini-xylA"
                     /product="hypothetical protein"
                     /label=mini-xylA
                     /note="mini xylA ORF"
                     /protein_id="APU90170.1"
                     /translation="MAQSHSSSVNYFVSVNKVV"
     gene            complement(8304..8363)
                     /gene="mini-xylA"
                     /label=mini-xylA
                     /note="xylA"
     protein_bind    complement(8433..8443)
                     /label=XylR binding site
                     /bound_moiety="XylR"
                     /note="xylO operator"
     protein_bind    complement(8447..8457)
                     /label=XylR binding site
                     /bound_moiety="XylR"
                     /note="xylO operator"
     regulatory      complement(8467..8472)
                     /regulatory_class="minus_10_signal"
                     /note="-10 (sigA)"
     regulatory      complement(8490..8497)
                     /regulatory_class="minus_35_signal"
                     /note="-35 (sigA)"
     CDS             8724..9776
                     /codon_start=1
                     /gene="xylR"
                     /product="XylR Xylose Repressor protein"
                     /label=xylR
                     /note="XylR"
                     /protein_id="APU90171.1"
                     /translation="MTGLNKSTVSSQVNTLMKENLVFEIGQGQSSGGRRPVMLVFNKKA
                     GYSIGIDVGVDYISGILTDLEGTIILDQHHHLESNSPEITKDILIDMIHHFITRMPQSP
                     YGLIGIGICVPGLIDKNQKIVFTPNSNWRDIDLKSFIQEKFNVPVFIENEANAGAYGEK
                     VFGAAKNHNNIIYASISTGIGIGVIINNHLYRGVSGFSGEMGHMTIDFNGPKCSCGNRG
                     CWELYASEKALLKSLQTKEKKVSYQDIIDLAHLNDIGTLNALQNFGFYLGIGLTNILNT
                     FNPQAIILRNSIIESHPMVLNSIRSEVSSRVYPQLGNSYELLPSSLGKNAPALGMSSIV
                     IEHFLDIVKM"
     gene            8724..9776
                     /gene="xylR"
                     /label=xylR
     misc_feature    9784..10683
                     /pseudo
                     /note="amyE; amyE front; amyE homology for integration into
                     B. amyloliquefaciens"