LOCUS       Exported                5607 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJG030, complete sequence.
ACCESSION   KX499660
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Goodson J, Klupt S, Zhang C, Straight P, Winkler W.
  TITLE     A Broadly Conserved Antiterminator Protein Controls a Regulon of 
            Bacillus amyloliquefaciens Antibiotic Gene Clusters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   Goodson JR.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2016) Cell Biology and Molecular Genetics, 
            University of Maryland, 3112 Bioscience Research Building, College 
            Park, MD 20742, USA
REFERENCE   3  (bases 1 to 5607)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5607
                     /organism="Cloning vector pJG030"
                     /mol_type="other DNA"
                     /country="USA"
                     /note="for ectopic integration into the Bacillus 
                     amyloliquefaciens chromosome."
                     /db_xref="taxon:1930832"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    397..1294
                     /note="LoaP region homology; homology region upstream of 
                     loaP containing dfnA leader"
     gene            complement(397..528)
                     /pseudo
                     /gene="dfnA"
                     /label=dfnA
     gene            1262..1294
                     /pseudo
                     /gene="loaP"
                     /label=loaP
                     /note="5"
     CDS             1745..2479
                     /codon_start=1
                     /gene="erm"
                     /product="hypothetical protein"
                     /label=erm
                     /note="ErmR"
                     /protein_id="APU90156.1"
                     /translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFT
                     LELVKRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIYGNIPY
                     NISTDIIRKIVFDSIANEIYLIVEYGFAKRLLNTKRSLALLLMAEVDISILSMVPREYF
                     HPKPKVNSSLIRLSRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGID
                     DLNNISFEQFLSLFNSYKLFNK"
     gene            1745..2479
                     /gene="erm"
                     /label=erm
     misc_feature    2573..3342
                     /note="LoaP Region Homology; homology region downstream of 
                     loaP"
     gene            2573..2603
                     /pseudo
                     /gene="loaP"
                     /label=loaP
                     /note="3"
     gene            complement(2947..3342)
                     /pseudo
                     /locus_tag="RBA_022100"
                     /label=RBA_022100
     misc_feature    3343..3373
                     /note="MCS; pUC19 multiple cloning site"
     primer_bind     complement(3386..3402)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3410..3426
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator; The lac repressor binds to the lac 
                     operator to inhibit transcription in E. coli. This 
                     inhibition can be relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      complement(3434..3464)
                     /regulatory_class="promoter"
                     /note="lac promoter; promoter for the E. coli lac operon"
     promoter        complement(3434..3464)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3479..3500
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3788..4376)
                     /direction=LEFT
                     /note="ori; high-copy-number ColE1/pMB1/pBR322/pUC origin 
                     of replication"
     CDS             complement(4547..5407)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR; confers resistance to ampicillin, 
                     carbenicillin, and related antibiotics"
                     /protein_id="APU90157.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(4547..5407)
                     /gene="bla"
                     /label=bla
     regulatory      complement(5408..5512)
                     /regulatory_class="promoter"
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        complement(5408..5512)
                     /gene="bla"
                     /label=AmpR promoter