LOCUS       Exported                7911 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJG019, complete sequence.
ACCESSION   KX499653
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7911)
  AUTHORS   Goodson J, Klupt S, Zhang C, Straight P, Winkler W.
  TITLE     A Broadly Conserved Antiterminator Protein Controls a Regulon of 
            Bacillus amyloliquefaciens Antibiotic Gene Clusters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7911)
  AUTHORS   Goodson JR.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2016) Cell Biology and Molecular Genetics, 
            University of Maryland, 3112 Bioscience Research Building, College 
            Park, MD 20742, USA
REFERENCE   3  (bases 1 to 7911)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7911
                     /organism="Cloning vector pJG019"
                     /mol_type="other DNA"
                     /country="USA"
                     /note="for ectopic integration into the Bacillus subtilis 
                     chromosome."
                     /db_xref="taxon:1930831"
     gene            complement(6..1034)
                     /pseudo
                     /gene="amyE"
                     /label=amyE
                     /note="amyE-back; 3 end of amyE homology"
     CDS             complement(1405..2055)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /note="CAT"
                     /protein_id="APU90121.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     gene            complement(1405..2055)
                     /gene="cat"
                     /label=cat
     regulatory      2368..2526
                     /regulatory_class="promoter"
                     /gene="yfp"
                     /note="Pconst"
     regulatory      2533..2543
                     /regulatory_class="ribosome_binding_site"
                     /gene="yfp"
                     /note="yfp RBS"
     CDS             2551..3267
                     /codon_start=1
                     /gene="yfp"
                     /product="yellow fluorescent protein"
                     /label=yfp
                     /note="YFP"
                     /protein_id="APU90122.1"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     gene            2551..3267
                     /gene="yfp"
                     /label=yfp
     regulatory      3274..3307
                     /regulatory_class="terminator"
                     /note="YFP_terminator"
     gene            complement(3315..3835)
                     /pseudo
                     /gene="amyE"
                     /label=amyE
                     /note="amyE-front; 5 end of amyE homology"
     regulatory      4055..4159
                     /regulatory_class="promoter"
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        4055..4159
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4160..5020
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR; confers resistance to ampicillin, 
                     carbenicillin, and related antibiotics"
                     /protein_id="APU90123.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            4160..5020
                     /gene="bla"
                     /label=bla
     rep_origin      5191..5779
                     /note="ori; high-copy-number ColE1/pMB1/pBR322/pUC origin 
                     of replication"
     CDS             complement(6026..6811)
                     /codon_start=1
                     /gene="spc"
                     /product="spectinomycin 3-adenyltransferase"
                     /label=spc
                     /note="SPC"
                     /protein_id="APU90124.1"
                     /translation="MMSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYPP
                     KREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLTDI
                     RRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEHVTL
                     LDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(6026..6811)
                     /gene="spc"
                     /label=spc