LOCUS       Exported                9512 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJFR3, complete sequence.
ACCESSION   KX011466
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9512)
  AUTHORS   Fernandez-Rodriguez J, Moser F, Voigt CA.
  TITLE     Multichromatic Bacterial Photography
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9512)
  AUTHORS   Fernandez-Rodriguez J, Moser F, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2016) Biological Engineering, MIT, 500 Tech 
            Square, Rm 140, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 9512)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9512
                     /organism="Cloning vector pJFR3"
                     /mol_type="other DNA"
                     /db_xref="taxon:1978234"
     misc_feature    231..278
                     /label=Terminator L3S1P22
                     /note="Terminator L3S1P22"
     CDS             complement(285..1031)
                     /codon_start=1
                     /product="pcyA"
                     /label=pcyA
                     /protein_id="ARG47676.1"
                     /translation="MAVTDLSLTNSSLMPTLNPMIQQLALAIAASWQSLPLKPYQLPED
                     LGYVEGRLEGEKLVIENRCYQTPQFRKMHLELAKVGKWLDILHCVMFPEPLYGLPLFGC
                     DIVAGPGGVSAAIADLSPTQSDRQLPAAYQKSLAELGQPEFEQQRELPPWGEIFSEYCL
                     FIRPSNVTEEERFVQRVVDFLQIHCHQSIVAEPLSEAQTLEHRQGQIHYCQQQQKNDKT
                     RRVLEKAFGEAWAERYMSQVLFDVIQ"
     RBS             1038..1049
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             complement(1052..1774)
                     /codon_start=1
                     /product="ho1"
                     /label=ho1
                     /protein_id="ARG47677.1"
                     /translation="MSVNLASQLREGTKKSHSMAENVGFVKCFLKGVVEKNSYRKLVGN
                     LYFVYSAMEEEMAKFKDHPILSHIYFPELNRKQSLEQDLQFYYGSNWRQEVKISAAGQA
                     YVDRVRQVAATAPELLVAHSYTRYLGDLSGGQILKKIAQNAMNLHDGGTAFYEFADIDD
                     EKAFKNTYRQAMNDLPINQATAERIVDEANDAFAMNMKMFNELEGNLIKAIGIMVFNSL
                     TRRRSQGSTEVGLATSEG"
     RBS             1781..1792
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     regulatory      complement(1809..1843)
                     /regulatory_class="promoter"
                     /note="Promoter J23108"
     promoter        complement(1809..1842)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     regulatory      1894..3050
                     /regulatory_class="promoter"
                     /note="Promoter PompC1157"
     CDS             3083..3829
                     /codon_start=1
                     /product="lambda CI_LVA"
                     /label=lambda CI_LVA
                     /protein_id="ARG47678.1"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFGAANDENYALVA"
     CDS             3083..3793
                     /codon_start=1
                     /gene="cI"
                     /product="phage lambda repressor"
                     /label=-lambda repressor
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     misc_feature    3794..3829
                     /label=similar to LVA tag
                     /note="similar to LVA tag"
     CDS             3794..3826
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     misc_feature    3863..4026
                     /label=Terminator DT11
                     /note="Terminator DT11"
     misc_feature    4065..4086
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     regulatory      4087..4168
                     /regulatory_class="promoter"
                     /note="Promoter Bba_J64067"
     misc_feature    4208..4333
                     /label=similar to SynZip 18
                     /note="similar to SynZip 18"
     misc_feature    4334..4348
                     /label=similar to GGSGG linker
                     /note="similar to GGSGG linker"
     misc_feature    4349..5203
                     /label=similar to K1F Fragment of T7 RNAP
                     /note="similar to K1F Fragment of T7 RNAP"
     misc_feature    5213..5259
                     /label=Terminator L3S3P11
                     /note="Terminator L3S3P11"
     terminator      5378..5407
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     misc_feature    complement(5455..5526)
                     /label=Terminator Bba_B0053
                     /note="Terminator Bba_B0053"
     misc_feature    5548..5578
                     /label=Terminator tR2
                     /note="Terminator tR2"
     CDS             complement(5592..5828)
                     /codon_start=1
                     /product="TrimR"
                     /label=TrimR
                     /protein_id="ARG47679.1"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     regulatory      complement(5856..5890)
                     /regulatory_class="promoter"
                     /note="Promoter Bba_J23119"
     promoter        complement(5856..5890)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     misc_feature    complement(6024..6548)
                     /label=nuc1
                     /note="nuc1"
     misc_feature    complement(6545..7174)
                     /label=kfrA
                     /note="kfrA"
     misc_feature    complement(7281..7801)
                     /label=repA
                     /note="repA"
     rep_origin      7420..7855
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     CDS             complement(7892..8863)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein of plasmid pSa"
                     /label=RepA
                     /translation="MPKNNKAPGHRINEIIKTSLALEMEDAREAGLVGYMARCLVQATM
                     PHTDPKTSYFERTNGIVTLSIMGKPSIGLPYGSMPRTLLAWICTEAVRTKDPVLNLGRS
                     QSEFLQRLGMHTDGRYTATLRNQAQRLFSSMISLAGEQGNDFGIENVVIAKRAFLFWNP
                     KRPEDRALWDSTLTLTGDFFEEVTRSPVPIRIDYLHALRQSPLAMDIYTWLTYRVFLLR
                     AKGRPFVQIPWVALQAQFGSSYGSRARNSPELDDKARERAERAALASFKYNFKKRLREV
                     LIVYPEASDCIEDDGECLRIKSTRLHVTRAPGKGARIGPPPT"
     misc_feature    complement(8856..9512)
                     /label=resP
                     /note="resP"