LOCUS       Exported                5846 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJFR2, complete sequence.
ACCESSION   KX011465
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Fernandez-Rodriguez J, Moser F, Voigt CA.
  TITLE     Multichromatic Bacterial Photography
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   Fernandez-Rodriguez J, Moser F, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2016) Biological Engineering, MIT, 500 Tech 
            Square, Rm 140, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 5846)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Cloning vector pJFR2"
                     /mol_type="other DNA"
                     /db_xref="taxon:1978233"
     regulatory      46..292
                     /regulatory_class="promoter"
                     /note="Promoter FixK2"
     CDS             326..928
                     /codon_start=1
                     /product="PhlF"
                     /label=PhlF
                     /protein_id="ARG47674.1"
                     /translation="MARTPSRSSIGSLRSPHTHKAILTSTIEILKECGYSGLSIESVAR
                     RAGASKPTIYRWWTNKAALIAEVYENESEQVRKFPDLGSFKADLDFLLRNLWKVWRETI
                     CGEAFRCVIAEAQLDPATLTQLKDQFMERRREMPKKLVENAISNGELPKDTNRELLLDM
                     IFGFCWYRLLTEQLTVEQDIEEFTFLLINGVCPGTQR"
     misc_feature    944..1072
                     /label=Terminator BBa_B0015
                     /note="Terminator BBa_B0015"
     terminator      952..1023
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1039..1066
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    1109..1138
                     /label=Operator PhlF
                     /note="Operator PhlF"
     misc_feature    1145..1219
                     /label=Insulator riboJ
                     /note="Insulator riboJ"
     misc_RNA        1147..1196
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     misc_feature    1225..1350
                     /label=similar to SynZip18
                     /note="similar to SynZip18"
     misc_feature    1351..1365
                     /label=similar to Linker GGSGG
                     /note="similar to Linker GGSGG"
     misc_feature    1366..2220
                     /label=similar to T3 fragment of T7 RNAP
                     /note="similar to T3 fragment of T7 RNAP"
     misc_feature    2230..2276
                     /label=Terminator L3S3P11
                     /note="Terminator L3S3P11"
     misc_feature    2342..2485
                     /label=Terminator DT25
                     /note="Terminator DT25"
     misc_feature    complement(2542..3396)
                     /label=similar to CGG fragment of T7 RNAP
                     /note="similar to CGG fragment of T7 RNAP"
     misc_feature    complement(3397..3411)
                     /label=similar to Linker GGSGG
                     /note="similar to Linker GGSGG"
     misc_feature    3412..3537
                     /label=similar to SynZip 18
                     /note="similar to SynZip 18"
     misc_feature    3579..3629
                     /label=Insulator BydvJ
                     /note="Insulator BydvJ"
     regulatory      3632..3803
                     /regulatory_class="promoter"
                     /note="Promoter PcpcG2"
     misc_feature    3938..4042
                     /label=Terminator T1
                     /note="Terminator T1"
     terminator      3943..4029
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      3955..4760
                     /label=p15A origin of replication
                     /note="p15A origin of replication"
     rep_origin      complement(4198..4743)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     misc_feature    4849..4899
                     /label=Terminator L3S1P13
                     /note="Terminator L3S1P13"
     CDS             complement(4907..5698)
                     /codon_start=1
                     /product="specR"
                     /label=specR
                     /protein_id="ARG47675.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     regulatory      5699..5846
                     /regulatory_class="promoter"