LOCUS       Exported               11252 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJFR1, complete sequence.
ACCESSION   KX011464
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11252)
  AUTHORS   Fernandez-Rodriguez J, Moser F, Voigt CA.
  TITLE     Multichromatic Bacterial Photography
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11252)
  AUTHORS   Fernandez-Rodriguez J, Moser F, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2016) Biological Engineering, MIT, 500 Tech 
            Square, Rm 140, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 11252)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11252
                     /organism="Cloning vector pJFR1"
                     /mol_type="other DNA"
                     /db_xref="taxon:1978232"
     misc_feature    1..61
                     /label=Terminator L3S2P21
                     /note="Terminator L3S2P21"
     misc_feature    87..107
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     misc_feature    complement(108..236)
                     /label=similar to SynZip 17
                     /note="similar to SynZip 17"
     misc_feature    complement(237..257)
                     /label=GGSGGGS linker
                     /note="GGSGGGS linker"
     misc_feature    complement(258..2057)
                     /label=similar to T7 RNA Polymerase core
                     /note="similar to T7 RNA Polymerase core"
     regulatory      2088..2122
                     /regulatory_class="promoter"
                     /note="Promoter J23105"
     misc_feature    2172..2228
                     /label=Terminator ECK120033737
                     /note="Terminator ECK120033737"
     CDS             complement(2241..2945)
                     /codon_start=1
                     /product="ccaR"
                     /label=ccaR
                     /protein_id="ARG47669.1"
                     /translation="MRILLVEDDLPLAETLAEALSDQLYTVDIATDASLAWDYASRLEY
                     DLVILDVMLPELDGITLCQKWRSHSYLMPILMMTARDTINDKITGLDAGADDYVVKPVD
                     LGELFARVRALLRRGCATCQPVLEWGPIRLDPSTYEVSYDNEVLSLTRKEYSILELLLR
                     NGRRVLSRSMIIDSIWKLESPPEEDTVKVHVRSLRQKLKSAGLSADAIETVHGIGYRLA
                     NLTEKSLCQGKN"
     CDS             complement(2986..5247)
                     /codon_start=1
                     /product="ccaS"
                     /label=ccaS
                     /protein_id="ARG47670.1"
                     /translation="MGKFLIPIEFVFLAIAMTCYLWHRQNQERRRIEISIKQQTQRERF
                     INQITQHIRQSLNLETVLNTTVAEVKTLLQVDRVLIYRIWQDGTGSAITESVNANYPSI
                     LGRTFSDEVFPVEYHQAYTKGKVRAINDIDQDDIEICLADFVKQFGVKSKLVVPILQHN
                     RASSLDNESEFPYLWGLLITHQCAFTRPWQPWEVELMKQLANQVAIAIQQSELYEQLQQ
                     LNKDLENRVEKRTQQLAATNQSLRMEISERQKTEAALRHTNHTLQSLIAASPRGIFTLN
                     LADQIQIWNPTAERIFGWTETEIIAHPELLTSNILLEDYQQFKQKVLSGMVSPSLELKC
                     QKKDGSWIEIVLSAAPLLDSEENIAGLVAVVADITEQKRQAEQIRLLQSVVVNTNDAVV
                     ITEAEPIDDPGPRILYVNEAFTKITGYTAEEMLGKTPRVLQGPKTSRTELDRVRQAISQ
                     WQSVTVEVINYRKDGSEFWVEFSLVPVANKTGFYTHWIAVQRDVTERRRTEEVRLALER
                     EKELSRLKTRFFSMASHEFRTPLSTALAAAQLLENSEVAWLDPDKRSRNLHRIQNSVKN
                     MVQLLDDILIINRAEAGKLEFNPNWLDLKLLFQQFIEEIQLSVSDQYYFDFICSAQDTK
                     ALVDERLVRSILSNLLSNAIKYSPGGGQIKIALSLDSEQIIFEVTDQGIGISPEDQKQI
                     FEPFHRGKNVRNITGTGLGLMVAKKCVDLHSGSILLKSAVDQGTTVTICLKRYNHLPRA
                     "
     regulatory      5283..5317
                     /regulatory_class="promoter"
                     /note="Promoter J23108"
     promoter        complement(5283..5316)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     regulatory      5363..5397
                     /regulatory_class="promoter"
                     /note="Promoter J23106"
     CDS             5428..7662
                     /codon_start=1
                     /product="cph8 (G722V)"
                     /label=cph8
                     /protein_id="ARG47671.1"
                     /translation="MATTVQLSDQSLRQLETLAIHTAHLIQPHGLVVVLQEPDLTISQI
                     SANCTGILGRSPEDLLGRTLGEVFDSFQIDPIQSRLTAGQISSLNPSKLWARVMGDDFV
                     IFDGVFHRNSDGLLVCELEPAYTSDNLPFLGFYHMANAALNRLRQQANLRDFYDVIVEE
                     VRRMTGFDRVMLYRFDENNHGDVIAEDKRDDMEPYLGLHYPESDIPQPARRLFIHNPIR
                     VIPDVYGVAVPLTPAVNPSTNRAVDLTESILRSAYHCHLTYLKNMGVGASLTISLIKDG
                     HLWGLIACHHQTPKVIPFELRKACEFFGRVVFSNISAQEDTETFDYRVQLAEHEAVLLD
                     KMTTAADFVEGLTNHPDRLLGLTGSQGAAICFGEKLILVGETPDEKAVQYLLQWLENRE
                     VQDVFFTSSLSQIYPDAVNFKSVASGLLAIPIARHNFLLWFRPEVLQTVNWGGDPNHAY
                     EATQEDGKIELHPRQSFDLWKEIVRLQSLPWQSVEIQSALALKKAIVNLILRQAEELHM
                     AAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEECNAIIE
                     QFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHPLSIKRAV
                     ANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVRGDSARTIS
                     GTGLGLAIVQRIVDNHNGMLELGTSERVGLSIRAWLPVPVTRAQGTTKEG"
     misc_feature    7669..7725
                     /label=Terminator L3S2P11
                     /note="Terminator L3S2P11"
     promoter        7751..7828
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     regulatory      7766..7802
                     /regulatory_class="promoter"
                     /note="Promoter LacIq35"
     misc_feature    7829..8954
                     /label=similar to YF1
                     /note="similar to YF1"
     CDS             8968..9585
                     /codon_start=1
                     /product="fixJ"
                     /label=fixJ
                     /protein_id="ARG47672.1"
                     /translation="MTTKGHIYVIDDDAAMRDSLNFLLDSAGFGVTLFDDAQAFLDALP
                     GLSFGCVVSDVRMPGLDGIELLKRMKAQQSPFPILIMTGHGDVPLAVEAMKLGAVDFLE
                     KPFEDDRLTAMIESAIRQAEPAAKSEAVAQDIAARVASLSPRERQVMEGLIAGLSNKLI
                     AREYDISPRTIEVYRANVMTKMQANSLSELVRLAMRAGMLND"
     misc_feature    9595..9651
                     /label=Terminator L3S2P55
                     /note="Terminator L3S2P55"
     rep_origin      9658..10340
                     /label=colE1 origin
                     /note="colE1 origin"
     rep_origin      complement(9676..10264)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    10345..10451
                     /label=Terminator T0
                     /note="Terminator T0"
     terminator      10352..10446
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(10467..11126)
                     /codon_start=1
                     /product="camR"
                     /label=camR
                     /protein_id="ARG47673.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"