LOCUS       Exported                3128 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Positive selection cloning vector pJET1, complete sequence.
ACCESSION   DQ317600
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Lubys A, Pagarauskaite K.
  TITLE     Positive selection cloning vector pJET1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Lubys A, Pagarauskaite K.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2005) R&D Department, Fermentas UAB, Graiciuno 8, 
            Vilnius LT-02241, Lithuania
REFERENCE   3  (bases 1 to 3128)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Positive selection cloning vector pJET1"
                     /mol_type="other DNA"
                     /db_xref="taxon:363226"
     CDS             complement(187..879)
                     /codon_start=1
                     /gene="eco47IR"
                     /product="restriction endonuclease Eco47I"
                     /label=eco47IR
                     /note="restriction endonuclease; in the absence of 
                     protective methylation the enzyme recognizes and cleaves 
                     double-stranded DNA targets 5-GGWCC-3"
                     /protein_id="ABC47403.1"
                     /translation="MSKETSFVKNAEELAKQKMDAINPELSSKFKFLIKFLSQFPEACS
                     KPRSKKMQNKVGQEEHIEYLARSFHESRLPRKPTPPTTVPDEVVSIVLNISFNIQPENL
                     ERIKEEHRFSMAAENIVGDLLERYLAEKLEPSGWIWCSGTSVKAVDFIHYDEKNNEWNL
                     LQVKNRDNTENSSSSKIRDNTTIKKWFRTYSQRDATNWDNFPDEVSSKNLNEEDFRAFV
                     KNYLAKII"
     gene            complement(187..879)
                     /gene="eco47IR"
                     /label=eco47IR
     protein_bind    908..924
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(932..962)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    977..998
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      1294..1908
                     /note="replication region from pMB1 (the same origin of 
                     replication is harbored by plasmids of pUC-series)"
     rep_origin      complement(1309..1897)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2068..2928)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="catalyzes the opening and hydrolysis of the 
                     beta-lactam ring of beta-lactam antibiotics such as 
                     penicillins and cephalosporins"
                     /protein_id="ABC47404.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2068..2928)
                     /gene="bla"
                     /label=bla
     promoter        complement(2929..3033)
                     /gene="bla"
                     /label=AmpR promoter