LOCUS       Exported               13009 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Bacterial expression vector pJE202, complete sequence.
ACCESSION   LT727306
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13009)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13009)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 13009)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13009
                     /organism="Bacterial expression vector pJE202"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 4113."
                     /db_xref="taxon:1944684"
     promoter        10..38
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             complement(866..1618)
                     /codon_start=1
                     /note="unnamed protein product; luxR"
                     /protein_id="SJL88050.1"
                     /translation="MKNINADDTYRIINKIKACRSNNDINQCLSDMTKMVHCEYYLLAI
                     IYPHSMVKSDISILDNYPKKWRQYYDDANLIKYDPIVDYSNSNHSPINWNIFENNAVNK
                     KSPNVIKEAKTSGLITGFSFPIHTANNGFGMLSFAHSEKDNYIDSLFLHACMNIPLIVP
                     SLVDNYRKINIANNKSNNDLTKREKECLAWACEGKSSWDISKILGCSERTVTFHLTNAQ
                     MKLNTTNRCQSISKAILTGAIDCPYFKN"
     CDS             1837..2418
                     /codon_start=1
                     /note="unnamed protein product; luxI"
                     /protein_id="SJL88051.1"
                     /translation="MTIMIKKSDFLAIPSEEYKGILSLRYQVFKQRLEWDLVVENNLES
                     DEYDNSNAEYIYACDDTENVSGCWRLLPTTGDYMLKSVFPELLGQQSAPKDPNIVELSR
                     FAVGKNSSKINNSASEITMKLFEAIYKHAVSQGITEYVTVTSTAIERFLKRIKVPCHRI
                     GDKEIHVLGDTKSVVLSMPINEQFKKAVLN"
     CDS             2472..3911
                     /codon_start=1
                     /note="unnamed protein product; luxC"
                     /protein_id="SJL88052.1"
                     /translation="MNKCIPMIINGMIQDFDNYAYKEVKLNNDNRVKLSVITESSVSKT
                     LNIKDRINLNLNQIVNFLYTVGQRWKSEEYNRRRTYIRELKTYLGYSDEMARLEANWIA
                     MLLCSKSALYDIVNYDLGSIHVLDEWLPRGDCYVKAQPKGVSVHLLAGNVPLSGVTSIL
                     RAILTKNECIIKTSSSDPFTANALVSSFIDVNADHPITKSMSVMYWPHDEDMTLSQRIM
                     NHADVVIAWGGDEAIKWAVKYSPPHVDILKFGPKKSLSIIEAPKDIEAAAMGVAHDICF
                     YDQQACFSTQDVYYIGDNLPLFLNELEKQLDRYAKILPKGSNSFDEKAAFTLTEKESLF
                     AGYEVRKGDKQAWLIVVSPTNSFGNQPLSRSVYVHQVSDIKEIIPFVNKNRTQTVSIYP
                     WEASLKYRDKLARSGVERIVESGMNNIFRVGGAHDSLSPLQYLVRFVSHERPFNYTTKD
                     VAVEIEQTRYLEEDKFLVFVP"
     CDS             3926..4849
                     /codon_start=1
                     /note="unnamed protein product; luxD"
                     /protein_id="SJL88053.1"
                     /translation="MKDESAFFTIDHIIKLDNGQSIRVWETLPKKNVPEKKHTILIASG
                     FARRMDHFAGLAEYLSTNGFHVIRYDSLHHVGLSSGCINEFTMSIGKNSLLTVVDWLTD
                     HGVERIGLIAASLSARIAYEVVNKIKLSFLITAVGVVNLRDTLEKALEYDYLQLPISEL
                     PEDLDFEGHNLGSEVFVTDCFKHNWDTLDSTLNSVKGLAIPFIAFTANDDSWVKQSEVI
                     ELIDSIESSNCKLYSLIGSSHDLGENLVVLRNFYQSVTKAALALDDGLLDLEIDIIEPR
                     FEDVTSITVKERRLKNEIENELLELA"
     CDS             4880..5944
                     /codon_start=1
                     /note="unnamed protein product; luxA"
                     /protein_id="SJL88054.1"
                     /translation="MKFGNICFSYQPPGETHKQVMDRFVRLGIASEEVGFDTYWTLEHH
                     FTEFGLTGNLFVAAANLLGRTKTLNVGTMGVVIPTAHPVRQLEDVLLLDQMSKGRFNFG
                     TVRGLYHKDFRVFGVDMEESRAITQNFYQMIMESLQTGTISSDSDYIQFPKVDVYPKVY
                     SKNVPTCMTAESASTTEWLAIQGLPMVLSWIIGTNEKKAQMELYNEIATEYGHDISKID
                     HCMTYICSVDDDAQKAQDVCREFLKNWYDSYVNATNIFNDSNQTRGYDYHKGQWRDFVL
                     QGHTNTNRRVDYSNGINPVGTPEQCIEIIQRDIDATGITNITCGFEANGTEDEIIASMR
                     RFMTQVAPFLKEPK"
     CDS             5983..6963
                     /codon_start=1
                     /note="unnamed protein product; luxB"
                     /protein_id="SJL88055.1"
                     /translation="MKFGLFFLNFQKDGITSEETLDNMVKTVTLIDSTKYHFNTAFVNE
                     HHFSKNGIVGAPITAAGFLLGLTNKLHIGSLNQVITTHHPVRVAEEASLLDQMSEGRFI
                     LGFSDCESDFEMEFFRRHISSRQQQFEACYEIINDALTTGYCHPQNDFYDFPKVSINPH
                     CYSENGPKQYVSATSKEVVMWAAKKALPLTFKWEDNLETKERYAILYNKTAQQYGIDIS
                     DVDHQLTVIANLNADRSTAQEEVREYLKDYITETYPQMDRDEKINCIIEENAVGSHDDY
                     YESTKLAVEKTGSKNILLSFESMSDIKDVKDIIDMLNQKIEMNLP"
     CDS             7038..8174
                     /codon_start=1
                     /note="unnamed protein product; luxE"
                     /protein_id="SJL88056.1"
                     /translation="MTVHTEYKRNQIIASSEIDDLIFMTKPQEWSFEEQKEIRDKLVRE
                     AFYFHYNRNEEYRNYCINQHVSDNLHTIDEIPVFPTSVFKYKKLHTVTAEDIENWYTSS
                     GTRGVKSHIARDRLSIERLLGSVNFGMKYVGDWFEHQMELINLGPDRFNTNNIWFKYVM
                     SLVELLYPTEFTVDNDKIDFEKTVKHLFRIKNSKKDICLIGPPFFVYLLCQYMKENNIE
                     FKGGDRVHIITGGGWKSNQNDSLDRADFNQLLMDTFQLDKINQIRDTFNQVELNTCFFE
                     DEFQRKHVPPWVYARALDPETLKPVADGEIGLLSYMDASSTAYPAFIVTDDIGIVKEIR
                     EPDPYPGVTVEIVRRLNTRAQKGCALSMANVIQKNIKD"
     CDS             8178..8888
                     /codon_start=1
                     /note="unnamed protein product; luxG"
                     /protein_id="SJL88057.1"
                     /translation="MIVDGRVSKIVLASIRNNIYKVFITVNSPIKFIAGQFVMVTINGK
                     KCPFSIANCPTKNYEIELHIGSSNRDCSLDIIEYFVDALVEEVAIELDAPHGNAWLRSE
                     SNNPLLLIAGGTGLSYINSILTNCLNRNIPQDIYLYWGVKDSSLLYEDEELLNLSLNNK
                     NFHYIPVIEDKSEEWIGRKGTVLDAVMEDFTDLTYFDIYVCGPFMMAKTAKEKLIEEKK
                     AKSEQMFADAFAYV"
     regulatory      8889..8928
                     /regulatory_class="terminator"
                     /note="luxICDABEG terminator"
     CDS             10563..10754
                     /codon_start=1
                     /note="unnamed protein product; rop"
                     /protein_id="SJL88058.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    10856..10996
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10998..11400)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(11182..11770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11941..12801)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin"
                     /protein_id="SJL88059.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12802..12906)
                     /gene="bla"
                     /label=AmpR promoter