LOCUS       Exported                5847 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pJDL, complete sequence.
ACCESSION   AF311601
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5847)
  AUTHORS   Park JB.
  TITLE     Concurrent measurement of promoter activity and transfection 
            efficiency using a new reporter vector containing both Photinus 
            pyralis and Renilla reniformis luciferase genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5847)
  AUTHORS   Park JB.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2000) PL, BHNRC, ARS, USDA, Barc-East, Bldg. 307, 
            Rm. 313, Beltsville, MD 20705, USA
REFERENCE   3  (bases 1 to 5847)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5847
                     /organism="Reporter vector pJDL"
                     /focus
                     /mol_type="genomic DNA"
                     /db_xref="taxon:152052"
     source          4034..5847
                     /organism="Cloning vector pUC18"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:72227"
     misc_feature    1..50
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             74..1726
                     /codon_start=1
                     /product="Photinus pyralis luciferase"
                     /label=Photinus pyralis luciferase
                     /note="firefly luciferase"
                     /protein_id="AAK09278.1"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     misc_feature    1743..1980
                     /label=contains SV40 early polyA_signal
                     /note="contains SV40 early polyA_signal"
     polyA_signal    1862..1983
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        2058..2361
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2362..2565
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          2631..2763
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        2808..2826
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2836..3771
                     /codon_start=1
                     /product="Renilla reniformis luciferase"
                     /label=Renilla reniformis luciferase
                     /protein_id="AAK09279.1"
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    3802..3923
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4056..4160
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4161..5021
                     /codon_start=1
                     /product="beta-lactamase"
                     /function="ampicillin resistance"
                     /label=beta-lactamase
                     /protein_id="AAK09280.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5192..5780
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"