LOCUS       Exported                6551 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  MTL KO vector pJD1, complete sequence.
ACCESSION   JX486681
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6551)
  AUTHORS   Lin CH, Kabrawala S, Fox EP, Nobile CJ, Johnson AD, Bennett RJ.
  TITLE     Genetic Control of Conventional and Pheromone-Stimulated Biofilm 
            Formation in Candida albicans
  JOURNAL   PLoS Pathog. 9 (4), E1003305 (2013)
  PUBMED    23637598
REFERENCE   2  (bases 1 to 6551)
  AUTHORS   Lohse MB.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-AUG-2012) Biochemistry and Biophysics, UCSF, Genentech
            Hall N374, 600 16th Street, San Francisco, CA 94143, USA
REFERENCE   3  (bases 1 to 6551)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6551
                     /organism="MTL KO vector pJD1"
                     /mol_type="other DNA"
                     /db_xref="taxon:1295330"
     misc_feature    7..530
                     /label=3flanking region of MTL
                     /note="3flanking region of MTL"
     primer_bind     531..550
                     /label=UP5 linker region
                     /note="UP5 linker region"
     CDS             1627..3021
                     /codon_start=1
                     /product="Arg4"
                     /EC_number="1.2.3.4"
                     /label=Arg4
                     /note="argininosuccinate lyase"
                     /protein_id="AGG09091.1"
                     /translation="MSQQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADLTGTK
                     VYTQGLNKLGLITTEELNLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGEIIGK
                     NISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMPGYTH
                     LQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREFLAKG
                     LGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLADAYS
                     TGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLFDALT
                     TVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISGECVR
                     KAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDAIGGTAKSAVLKQLEN
                     LKSILN"
     primer_bind     3838..3857
                     /label=UP2 linker region
                     /note="UP2 linker region"
     misc_feature    3858..4340
                     /label=5flanking region of MTL
                     /note="5flanking region of MTL"
     primer_bind     complement(4394..4410)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4418..4434
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4442..4472)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4487..4508
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      4778..5460
                     /label=ColE1 origin
                     /note="ColE1 origin"
     rep_origin      complement(4796..5384)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5555..6415)
                     /codon_start=1
                     /gene="BLA"
                     /product="ampicillin resistance protein"
                     /label=BLA
                     /protein_id="AGG09092.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(5555..6415)
                     /gene="BLA"
                     /label=BLA
     promoter        complement(6416..6520)
                     /gene="bla"
                     /label=AmpR promoter