LOCUS       Exported                3775 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Allelic replacement vector pJC84, complete sequence.
ACCESSION   FJ155667
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Wehrly TD, Chong A, Virtaneva K, Sturdevant DE, Child R, Edwards JA,
            Brouwer D, Nair V, Fischer ER, Wicke L, Curda AJ, Kupko JJ III, 
            Martens C, Crane DD, Bosio CM, Porcella SF, Celli J.
  TITLE     Intracellular biology and virulence determinants of Francisella 
            tularensis revealed by transcriptional profiling inside macrophages
  JOURNAL   Cell. Microbiol. 11 (7), 1128-1150 (2009)
  PUBMED    19388904
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Wehrly TD, Chong A, Virtaneva K, Sturdevant DE, Edwards JA, Nair V, 
            Fischer ER, Wicke L, Curda AJ, Kupko JJ III, Martens C, Crane DD, 
            Bosio CM, Porcella SF, Celli J.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-AUG-2008) Laboratory of Intracellular Parasites, 
            RML/NIAID/DIR/NIH, 903 South 4th street, Hamilton, MT 59840, USA
REFERENCE   3  (bases 1 to 3775)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Allelic replacement vector pJC84"
                     /mol_type="other DNA"
                     /db_xref="taxon:561109"
     CDS             complement(77..1498)
                     /codon_start=1
                     /gene="sacB"
                     /product="SacB"
                     /label=sacB
                     /protein_id="ACH99099.1"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     gene            complement(77..1498)
                     /gene="sacB"
                     /label=sacB
     CDS             complement(1519..2373)
                     /codon_start=1
                     /gene="aph"
                     /product="Aph"
                     /label=aph
                     /note="kanamycin resistance"
                     /protein_id="ACH99098.1"
                     /translation="MNIRPLQDRVLVRRARRSFRMIEQDGLHAGSPAAWVERLFGYDWA
                     QQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVV
                     TEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIER
                     ARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGR
                     FSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLD
                     EFF"
     gene            complement(1519..2373)
                     /gene="aph"
                     /label=aph
     CDS             complement(1519..2313)
                     /codon_start=1
                     /gene="aph(3)-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      complement(2389..2658)
                     /regulatory_class="promoter"
                     /note="Francisella tularensis groEL promoter"
     misc_feature    2659..2715
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        complement(2725..2743)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3001..3589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"