LOCUS       Exported                9019 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pJawohl8-RNAi, complete sequence.
ACCESSION   AF408413
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Ulker B, Lipka V, Rademacher TR, Somssich IE.
  TITLE     pJawohl8-RNAi a binary vector for gene silencing in plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9019)
  AUTHORS   Ulker B, Lipka V, Rademacher TR, Somssich IE.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2001) Biochemistry, Max-Planck-Institut 
            f.Zuechtungsforschung, Carl-von-Linne Weg 10, Koeln, NRW D-50829, 
            Germany
REFERENCE   3  (bases 1 to 9019)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9019
                     /organism="Binary vector pJawohl8-RNAi"
                     /focus
                     /mol_type="genomic DNA"
                     /note="binary plant gene silencing vector for one-step 
                     cloning of inverted sequences"
                     /db_xref="taxon:188084"
     source          3803..9019
                     /organism="Binary vector pJawohl3-RNAi"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:176105"
     repeat_region   26..1733
                     /note="sense orientation of Gateway conversion cassette 
                     frame A containing attR1-R2 repeats, CmR gene and ccdB 
                     gene"
     misc_feature    26..152
                     /label=attR1 of Gateway conversion cassette frame A
                     /note="attR1 of Gateway conversion cassette frame A"
     protein_bind    29..153
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        178..208
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             262..921
                     /codon_start=1
                     /gene="CmR"
                     /product="CmR"
                     /function="confers resistance to antibiotic 
                     chloramphenicol"
                     /label=CmR
                     /protein_id="AAM62300.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            262..921
                     /gene="CmR"
                     /label=CmR
     CDS             1263..1568
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB"
                     /label=ccdB
                     /note="encodes a cytotoxic protein that is a potent poison 
                     of DNA gyrase"
                     /protein_id="AAM62301.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            1263..1568
                     /gene="ccdB"
                     /label=ccdB
     protein_bind    complement(1609..1733)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    1610..1736
                     /label=attR2 of Gateway conversion cassette frame A
                     /note="attR2 of Gateway conversion cassette frame A"
     misc_feature    1762..2048
                     /note="contains intron 1 of Arabidopsis thaliana WRKY 
                     transcription factor 33"
     repeat_region   2073..3783
                     /note="antisense orientation of Gateway conversion cassette
                     frame A containing attR1-R2 repeats, CmR gene and ccdB 
                     gene"
     misc_feature    complement(2073..2199)
                     /label=attR2 of Gateway conversion cassette frame A
                     /note="attR2 of Gateway conversion cassette frame A"
     protein_bind    2076..2200
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2241..2546)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB"
                     /label=ccdB
                     /note="encodes a cytotoxic protein that is a potent poison 
                     of DNA gyrase"
                     /protein_id="AAM62303.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(2241..2546)
                     /gene="ccdB"
                     /label=ccdB
     CDS             complement(2888..3547)
                     /codon_start=1
                     /gene="CmR"
                     /product="CmR"
                     /function="confers resistance to antibiotic 
                     chloramphenicol"
                     /label=CmR
                     /protein_id="AAM62302.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            complement(2888..3547)
                     /gene="CmR"
                     /label=CmR
     promoter        complement(3601..3631)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(3656..3780)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    complement(3657..3783)
                     /label=attR1 of Gateway conversion cassette frame A
                     /note="attR1 of Gateway conversion cassette frame A"
     misc_feature    4097..4121
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      4233..4942
                     /label=oriV
                     /note="incP origin of replication"
     promoter        5016..5107
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5108..5968
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6139..6727
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    7039..7063
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      7157..7409
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7429..7980)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(8023..8202)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        8972..9018
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"