LOCUS       Exported                5597 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pJawohl3-RNAi, complete sequence.
ACCESSION   AF404854
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Ulker B, Lipka V, Rademacher TR, Somssich I.
  TITLE     pJawohl3-RNAi, a binary vector for gene silencing in plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Ulker B, Lipka V, Rademacher TR, Somssich I.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-2001) Biochemistry, Max-Planck-Institut f. 
            Zuechtungsforschung, Carl-von-Linne Weg 10, Koeln, NRW D-50829, 
            Germany
REFERENCE   3  (bases 1 to 5597)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Binary vector pJawohl3-RNAi"
                     /focus
                     /mol_type="genomic DNA"
                     /db_xref="taxon:176105"
     source          603..3662
                     /organism="Binary vector pPAM"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:155765"
     misc_feature    1..50
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    51..337
                     /note="Arabidopsis thaliana putative WRKY-type DNA-binding 
                     protein (transcription factor) intron presented in Genbank 
                     Accession Number AC005499"
     misc_feature    338..386
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    387..604
                     /note="Cauliflower mosaic virus 35S polyadenylation signal"
     misc_feature    675..699
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      811..1520
                     /label=oriV
                     /note="incP origin of replication"
     promoter        1594..1685
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1686..2546
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2717..3305
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3617..3641
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    complement(3716..4000)
                     /label=nopaline synthase (NOS) polyadenylation signal
                     /note="nopaline synthase (NOS) polyadenylation signal"
     terminator      3735..3987
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4007..4558)
                     /codon_start=1
                     /gene="pat"
                     /gene_synonym="bar"
                     /product="phosphinothricin acetyltransferase"
                     /function="confers resistance to herbicide BASTA"
                     /label=pat
                     /note="PAT; BAR"
                     /protein_id="AAL30502.1"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     gene            complement(4007..4558)
                     /gene="pat"
                     /gene_synonym="bar"
                     /label=pat
     regulatory      complement(4579..4889)
                     /regulatory_class="promoter"
                     /note="nopaline synthase (NOS) promoter"
     promoter        complement(4601..4780)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     regulatory      4900..5597
                     /regulatory_class="promoter"
                     /note="Cauliflower mosaic virus 35S gene promoter with 
                     duplicated enhancer region (CaMV35Sx2)"
     promoter        5550..5596
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"