LOCUS       Exported                6766 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJAP8, complete sequence.
ACCESSION   AY032976
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Tamura GS, Nittayajarn A, Schoentag DL.
  TITLE     A glutamine transport gene, glnQ, is required for fibronectin 
            adherence and virulence of group B streptococci
  JOURNAL   Infect. Immun. 70 (6), 2877-2885 (2002)
  PUBMED    12010975
REFERENCE   2  (bases 1 to 6766)
  AUTHORS   Tamura GS, Schoentag DL, Nittayajarn A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2001) Pediatrics, Childrens Hospital and Regional
            Medical Center and the University of Washington, 4800 Sand Point Way
            NE, Seattle, WA 98105-0371, USA
REFERENCE   3  (bases 1 to 6766)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6766
                     /organism="Cloning vector pJAP8"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:160803"
     promoter        complement(32..50)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(69..85)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    93..109
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(117..147)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    162..183
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(471..1059)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1230..2090)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="AAK62568.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2091..2195)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2222..2677
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     2819..2835
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2869..4470
                     /label=3 portion of glnP
                     /note="3 portion of glnP"
     CDS             4470..5210
                     /codon_start=1
                     /gene="glnQ"
                     /product="ATP binding protein"
                     /label=glnQ
                     /note="component of glutamine transport system"
                     /protein_id="AAK62567.1"
                     /translation="MAELKIDVQDLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGKS
                     TFLRTLNLLESITSGKVVVDGFELSNPKTDIDKARENIGMVFQHFNLFPHMSVLENITF
                     APIELGKESKEAAEKHGMELLEKVGLADKANAKPDSLSGGQKQRVAIARSLAMNPDILL
                     FDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGRFLEDG
                     TPEQIFDTPQHPRLQDFLNKVLNV"
     gene            4470..5210
                     /gene="glnQ"
                     /label=glnQ
     CDS             complement(4952..4969)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     CDS             5360..5710
                     /codon_start=1
                     /gene="ORFX"
                     /product="unknown protein"
                     /label=ORFX
                     /note="near glutamine transport operon"
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /protein_id="AAK62566.1"
                     /translation="MKDYINRILHFIKEHMTYHVNFIDDFLDIKWEKVSNIHLRFWTTI
                     IAYLVIFILSISTVILNLVLLFQGFLTQNPIIYLLFFITLVCAFYFAYKFITYTPTIVK
                     NALQYIKKLKNV"
     gene            5360..5710
                     /gene="ORFX"
                     /label=ORFX