LOCUS       Exported                8317 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pJ3ECAD, complete sequence.
ACCESSION   LT727097
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8317)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8317)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8317)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8317
                     /organism="Mammalian expression vector pJ3ECAD"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 5155."
                     /db_xref="taxon:1945052"
     polyA_signal    1..135
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(61..157)
                     /regulatory_class="terminator"
                     /note="SV40 polyA early"
     regulatory      61..157
                     /regulatory_class="terminator"
                     /note="SV40 polyA late"
     misc_feature    complement(158..709)
                     /label=3 UTR of SV40 small t-antigen
                     /note="3 UTR of SV40 small t-antigen"
     CDS             complement(560..580)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    complement(710..776)
                     /label=intron SV40 small t-antigen
                     /note="intron SV40 small t-antigen"
     intron          710..775
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(777..851)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small 
                     t-antigen"
                     /protein_id="SJL87422.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     misc_feature    complement(985..2995)
                     /label=3 UTR of hCDH1
                     /note="3 UTR of hCDH1"
     CDS             complement(2996..5644)
                     /codon_start=1
                     /note="unnamed protein product; hCDH1"
                     /protein_id="SJL87423.1"
                     /translation="MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRH
                     LERGRVLGRVNFEDCTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWD
                     STYRKFSTKVTLNTVGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISC
                     PENEKGPFPKNLVQIKSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDR
                     ERIATYTLFSHAVSSNGNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSV
                     MEVTATDADDDVNTYNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPT
                     YTLVVQAADLQGEGLSTTATAVITVTDTNDNPPIFNPTTYKGQVPENEANVVITTLKVT
                     DADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTN
                     VVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFM
                     EQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVAT
                     GTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPNTSPFTAELTHGAS
                     ANWTIQYNDPTQESIILKPKMALEVGDYKINLKLMDNQNKDQVTTLEVSVCDCEGAAGV
                     CRKAQPVEAGLQIPAILGILGGILALLILILLLLLFLRRRAVVKEPLLPPEDDTRDNVY
                     YYDEEGGGEEDQDFDLSQLHRGLDARPEVTRNDVAPTLMSVPRYLPRPANPDEIGNFID
                     ENLKAADTDPTAPPYDSLLVFDYEGSGSEAASLSSLNSSESDKDQDYDYLNEWGNRFKK
                     LADMYGGGEDD"
     misc_feature    complement(5645..5738)
                     /label=5 UTR of hCDH1
                     /note="5 UTR of hCDH1"
     promoter        complement(5757..6086)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5771..5906
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(5782..5857)
                     /direction=LEFT
                     /label=SV40 ori
                     /note="SV40 ori"
     regulatory      complement(5814..6095)
                     /regulatory_class="promoter"
                     /note="SV40 early promoter"
     misc_feature    6237..6377
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6379..6781)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(6563..7151)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7322..8182)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL87424.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8183..8287)
                     /gene="bla"
                     /label=AmpR promoter