LOCUS       Exported                4594 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pIZT-W(Gm) DNA, complete sequence.
ACCESSION   AB711107
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Tatsuke T, Lee J, Iiyama K, Sezutsu H, Uchino K, Kusakabe T.
  TITLE     Tightly controlled Tetracycline-inducible transcription system for 
            explosive gene expression in cultured silkworm cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Tatsuke T, Kusakabe T.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2012) Contact:Takahiro Kusakabe Graduate School of
            Bioresource and Bioenvironmental Sciences, Kyushu University, 
            Laboratory of Silkworm Science; 6-10-1 Hakozaki, Higashi-ku, 
            Fukuoka, Fukuoka 812-8581, Japan
REFERENCE   3  (bases 1 to 4594)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Expression vector pIZT-W(Gm)"
                     /mol_type="other DNA"
                     /db_xref="taxon:1170624"
     regulatory      1..549
                     /regulatory_class="promoter"
                     /note="Orgyia pseudotsugata nuclear polyhedrosis virus IE2 
                     promoter"
     promoter        1..548
                     /gene="immediate-early gene 2 from the Orgyia pseudotsugata
                     multicapsid nucleopolyhedrovirus"
                     /label=OpIE-2 promoter
                     /note="strong constitutive baculovirus promoter for insect 
                     cell expression"
     misc_recomb     647..771
                     /label=attR1 recombination site
                     /note="attR1 recombination site"
     protein_bind    647..771
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        796..826
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             880..1539
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /protein_id="BAM10888.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            880..1539
                     /gene="cat"
                     /label=cat
     CDS             1881..2186
                     /codon_start=1
                     /gene="ccdB"
                     /product="cytotoxic protein CcdB"
                     /label=ccdB
                     /protein_id="BAM10889.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            1881..2186
                     /gene="ccdB"
                     /label=ccdB
     misc_recomb     2227..2351
                     /label=attR2 recombination site
                     /note="attR2 recombination site"
     protein_bind    complement(2227..2351)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2386..2427
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2437..2454
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    2472..2601
                     /note="Orgyia pseudotsugata nuclear polyhedrosis virus IE2 
                     polyadenylation signal"
     polyA_signal    2472..2601
                     /gene="immediate-early gene 2 from the Orgyia pseudotsugata
                     multicapsid nucleopolyhedrovirus"
                     /label=OpIE-2 poly(A) signal
                     /note="baculovirus polyadenylation signal"
     rep_origin      2670..3343
                     /label=pUC replication origin
                     /note="pUC replication origin"
     rep_origin      complement(2729..3317)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3773..3801
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             3990..4523
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyl transferase"
                     /label=aacC1
                     /protein_id="BAM10890.1"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     gene            3990..4523
                     /gene="aacC1"
                     /label=aacC1