LOCUS       Exported                4887 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pINV-110, complete sequence.
ACCESSION   AY952936
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Basu S, Gerchman Y, Collins CH, Arnold FH, Weiss R.
  TITLE     A synthetic multicellular system for programmed pattern formation
  JOURNAL   Nature 434 (7037), 1130-1134 (2005)
  PUBMED    15858574
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Basu S, Gerchman Y, Weiss R.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2005) Electrical Engineering, Princeton 
            University, B-312, E-Quad, Olden Street, Princeton, NJ 08544, USA
REFERENCE   3  (bases 1 to 4887)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Expression vector pINV-110"
                     /mol_type="other DNA"
                     /note="pLD; cyan reporter plasmid"
                     /db_xref="taxon:316975"
     CDS             complement(19..1101)
                     /codon_start=1
                     /gene="lacI"
                     /product="LacI"
                     /label=lacI
                     /note="repressor protein"
                     /protein_id="AAX29985.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     gene            complement(19..1101)
                     /gene="lacI"
                     /label=lacI
     promoter        complement(1102..1179)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     regulatory      complement(1128..1183)
                     /regulatory_class="promoter"
                     /note="PlacIq"
     regulatory      1233..1316
                     /regulatory_class="promoter"
                     /note="Plac"
     protein_bind    1245..1266
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        1281..1311
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1319..1335
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1367..1378
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1385..2098
                     /codon_start=1
                     /gene="cI"
                     /product="lambda repressor CI"
                     /label=cI
                     /protein_id="AAX29986.1"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     gene            1385..2098
                     /gene="cI"
                     /label=cI
     gene            2156..2893
                     /gene="ecfP"
                     /label=ecfP
     RBS             2156..2167
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     regulatory      2156..2164
                     /regulatory_class="ribosome_binding_site"
                     /gene="ecfP"
     CDS             2174..2893
                     /codon_start=1
                     /gene="ecfP"
                     /product="enhanced cyan fluorescent protein"
                     /label=ecfP
                     /note="EcfP"
                     /protein_id="AAX29988.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      2913..3017
                     /regulatory_class="terminator"
     terminator      2917..3003
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(3173..3718)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     regulatory      3827..3932
                     /regulatory_class="terminator"
     terminator      3832..3926
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3957..4751)
                     /codon_start=1
                     /gene="kanR"
                     /product="kanamycin resistance protein"
                     /label=kanR
                     /note="KanR; antibiotic resistance protein"
                     /protein_id="AAX29987.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            complement(3957..4751)
                     /gene="kanR"
                     /label=kanR