LOCUS       Exported                6394 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast integrative vector pINTyrD, complete sequence.
ACCESSION   EF202086
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   Mirisola MG, Colomba L, Gallo A, Amodeo R, De Leo G.
  TITLE     Yeast vectors for the integration/expression of any sequence at the 
            TYR1 locus
  JOURNAL   Yeast 24 (9), 761-766 (2007)
  PUBMED    17597490
REFERENCE   2  (bases 1 to 6394)
  AUTHORS   Mirisola MG.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2006) Biopathology, University of Palermo, via 
            Divisi, 83, Palermo 90100, Italy
REFERENCE   3  (bases 1 to 6394)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6394
                     /organism="Yeast integrative vector pINTyrD"
                     /mol_type="other DNA"
                     /db_xref="taxon:435645"
     misc_feature    59..290
                     /label=TYR1 recombination cassette
                     /note="TYR1 recombination cassette"
     misc_feature    295..2510
                     /label=LEU2 marker
                     /note="LEU2 marker"
     promoter        535..942
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             943..2037
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     regulatory      2518..3022
                     /regulatory_class="promoter"
                     /note="GAL10"
     promoter        2694..3024
                     /gene="S. cerevisiae GAL10"
                     /label=GAL10 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    2694..2811
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     regulatory      3059..3068
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3065..3784
                     /codon_start=1
                     /gene="GFP"
                     /product="green fluorescent protein"
                     /label=GFP
                     /protein_id="ABO93513.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     gene            3065..3784
                     /gene="GFP"
                     /label=GFP
     misc_feature    3811..4080
                     /label=TYR1 recombination cassette
                     /note="TYR1 recombination cassette"
     promoter        4488..4592
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4593..5453
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="ABO93514.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5624..6212
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"