LOCUS       Exported                4240 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast integrative vector pINTyrA, complete sequence.
ACCESSION   EF202083
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Mirisola MG, Colomba L, Gallo A, Amodeo R, De Leo G.
  TITLE     Yeast vectors for the integration/expression of any sequence at the 
            TYR1 locus
  JOURNAL   Yeast 24 (9), 761-766 (2007)
  PUBMED    17597490
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Mirisola MG.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2006) Biopathology, University of Palermo, via 
            Divisi, 83, Palermo 90100, Italy
REFERENCE   3  (bases 1 to 4240)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Yeast integrative vector pINTyrA"
                     /mol_type="other DNA"
                     /db_xref="taxon:435642"
     misc_feature    58..306
                     /label=TYR1 recombination cassette
                     /note="TYR1 recombination cassette"
     misc_feature    314..1225
                     /label=SUP16
                     /note="SUP16"
     misc_feature    1230..1927
                     /label=TYR1 recombination cassette
                     /note="TYR1 recombination cassette"
     promoter        2334..2438
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2439..3299
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="ABO93510.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3470..4058
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"