LOCUS       Exported                8008 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pINT, complete sequence.
ACCESSION   DQ813654
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8008)
  AUTHORS   Nkrumah LJ, Muhle RA, Moura PA, Ghosh P, Hatfull GF, Jacobs WR Jr., 
            Fidock DA.
  TITLE     Efficient site-specific integration in Plasmodium falciparum 
            chromosomes mediated by mycobacteriophage Bxb1 integrase
  JOURNAL   Nat. Methods 3 (8), 615-621 (2006)
  PUBMED    16862136
REFERENCE   2  (bases 1 to 8008)
  AUTHORS   Nkrumah LJ, Fidock DA.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2006) Microbiology and Immunology, Albert Einstein
            College of Medicine, 1300 Morris Park, Bronx, NY 10461, USA
REFERENCE   3  (bases 1 to 8008)
  AUTHORS   Nkrumah LJ, Fidock DA.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2007) Microbiology and Immunology, Albert Einstein
            College of Medicine, 1300 Morris Park, Bronx, NY 10461, USA
REFERENCE   4  (bases 1 to 8008)
  AUTHORS   Nkrumah LJ, Fidock DA.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2009) Microbiology and Immunology, Albert Einstein
            College of Medicine, 1300 Morris Park, Bronx, NY 10461, USA
REFERENCE   5  (bases 1 to 8008)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On Jul 10, 2009 this sequence version replaced DQ813654.2.
FEATURES             Location/Qualifiers
     source          1..8008
                     /organism="Cloning vector pINT"
                     /mol_type="other DNA"
                     /db_xref="taxon:393300"
     regulatory      complement(40..876)
                     /regulatory_class="terminator"
                     /note="derived from hsp86 3 UTR"
     CDS             complement(882..1682)
                     /codon_start=1
                     /gene="Neo"
                     /product="neomycin phosphotransferase II"
                     /label=Neo
                     /note="selectable marker"
                     /protein_id="ABG81368.1"
                     /translation="MSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            complement(882..1682)
                     /gene="Neo"
                     /label=Neo
     misc_feature    complement(1699..2544)
                     /label=contains promoter and 5 UTR from hsp86 gene
                     /note="contains promoter and 5 UTR from hsp86 gene"
     misc_feature    2552..3144
                     /label=contains promoter and 5 UTR from PcDT gene
                     /note="contains promoter and 5 UTR from PcDT gene"
     CDS             3161..4711
                     /codon_start=1
                     /product="integrase-myc"
                     /label=integrase-myc
                     /note="myc-tagged integrase; derived from mycobacteriophage
                     Bxb1"
                     /protein_id="ABG81369.1"
                     /translation="MRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLD
                     VSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLV
                     VSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGY
                     LPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQG
                     REPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAE
                     LVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTV
                     AMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAG
                     SPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSM
                     NVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMSRAAALEEQKLISEEDL"
     CDS             4679..4708
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      4718..5308
                     /regulatory_class="terminator"
                     /note="derived from hrp2 3 UTR"
     promoter        complement(5326..5343)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5350..5366)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5840..5944
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5945..6805
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6976..7564
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7852..7873
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7888..7918
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7926..7942
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7950..7966
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7984..8002
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"