LOCUS       Exported                3174 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pIGWZMCS, complete sequence.
ACCESSION   JN835257
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Zaleski P, Wawrzyniak P, Sobolewska A, Mikiewicz D, 
            Wojtowicz-Krawiec A, Chojnacka-Puchta L, Zielinski M, Plucienniczak 
            G, Plucienniczak A.
  TITLE     New cloning and expression vector derived from Escherichia coli 
            plasmid pIGWZ12; a potential vector for a two-plasmid expression 
            system
  JOURNAL   Plasmid 67 (3), 264-271 (2012)
  PUBMED    22230664
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Zaleski P, Plucienniczak G, Plucienniczak A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2011) Dept. of Bioengineering, Institute of 
            Biotechnology and Antibiotics, Staroscinska 5, Warsaw 02-516, Poland
REFERENCE   3  (bases 1 to 3174)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Cloning vector pIGWZMCS"
                     /mol_type="other DNA"
                     /db_xref="taxon:1148056"
     misc_feature    142..160
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     CDS             279..1094
                     /codon_start=1
                     /gene="kan"
                     /product="kanamycin resistance protein"
                     /label=kan
                     /protein_id="AFB74205.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            279..1094
                     /gene="kan"
                     /label=kan
     promoter        1359..1377
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    1396..1475
                     /label=multi-cloning site
                     /note="multi-cloning site"
     primer_bind     complement(1447..1463)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(1489..1507)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1514..1530)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2388..3080
                     /codon_start=1
                     /gene="rep"
                     /product="replication initation protein"
                     /label=rep
                     /protein_id="AFB74206.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
     gene            2388..3080
                     /gene="rep"
                     /label=rep