LOCUS       Exported                3072 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pIGPZA, complete sequence.
ACCESSION   JN900237
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Zaleski P, Wawrzyniak P, Sobolewska A, Mikiewicz D, 
            Wojtowicz-Krawiec A, Chojnacka-Puchta L, Zielinski M, Plucienniczak 
            G, Plucienniczak A.
  TITLE     New cloning and expression vector derived from Escherichia coli 
            plasmid pIGWZ12; a potential vector for a two-plasmid expression 
            system
  JOURNAL   Plasmid 67 (3), 264-271 (2012)
  PUBMED    22230664
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Zaleski P, Plucienniczak G, Plucienniczak A.
  TITLE     pIGPZA - a derivative of pIGPZ expression vector
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3072)
  AUTHORS   Zaleski P, Plucienniczak G, Plucienniczak A.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2011) Dept. of Bioengineering, Institute of 
            Biotechnology and Antibiotics, Staroscinska 5, Warsaw 02-516, Poland
REFERENCE   4  (bases 1 to 3072)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Cloning vector pIGPZA"
                     /mol_type="other DNA"
                     /db_xref="taxon:1156483"
     CDS             complement(153..1013)
                     /codon_start=1
                     /product="AmpR"
                     /label=AmpR
                     /note="ampicillin resistance"
                     /protein_id="AFC75628.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1014..1085)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1130..1148
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    complement(1161..1240)
                     /label=MCS
                     /note="MCS"
     primer_bind     complement(1218..1234)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(1245..1387)
                     /regulatory_class="promoter"
     promoter        complement(1396..1414)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1421..1437)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2295..2987
                     /codon_start=1
                     /product="replication protein"
                     /label=replication protein
                     /protein_id="AFC75629.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"