LOCUS       Exported               10781 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector piggyBac_PB, complete sequence.
ACCESSION   AY515146
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10781)
  AUTHORS   Thibault ST, Singer MA, Miyazaki WY, Milash B, Dompe NA, Singh CM, 
            Buchholz R, Demsky M, Fawcett R, Francis-Lang HL, Ryner L, Cheung 
            LM, Chong A, Erickson C, Fisher WW, Greer K, Hartouni SR, Howie E, 
            Jakkula L, Joo D, Killpack K, Laufer A, Mazzotta J, Smith RD, 
            Stevens LM, Stuber C, Tan LR, Ventura R, Woo A, Zakrajsek I, Zhao L,
            Chen F, Swimmer C, Kopczynski C, Duyk G, Winberg ML, Margolis J.
  TITLE     A complementary transposon tool kit for Drosophila melanogaster 
            using P and piggyBac
  JOURNAL   Nat. Genet. 36 (3), 283-287 (2004)
  PUBMED    14981521
REFERENCE   2  (bases 1 to 10781)
  AUTHORS   Thibault ST.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2003) Exelixis, Inc., 170 Harbor Way, PO Box 511, 
            South San Francisco, CA 94083, USA
REFERENCE   3  (bases 1 to 10781)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10781
                     /organism="Cloning vector piggyBac_PB"
                     /mol_type="other DNA"
                     /db_xref="taxon:261396"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             201..1061
                     /codon_start=1
                     /gene="Amp"
                     /product="beta-lactamase"
                     /function="ampicillin resistance"
                     /label=Amp
                     /protein_id="AAS09816.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            201..1061
                     /gene="Amp"
                     /label=Amp
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4498..4545
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(join(5211..5825,5896..6027,6231..6576,6609..7263,
                     7338..7638,7996..8067))
                     /codon_start=1
                     /gene="white"
                     /allele="+mC"
                     /product="white protein"
                     /label=white
                     /protein_id="AAS09817.1"
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNLIFEIPYHCRVTADASQSCIN
                     QGFGQAKNYGTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRT
                     RGLFCNERHIPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVS
                     PSGMRLLNGQPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVA
                     RVDQVIQELSLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLD
                     SFTAHSVVQVLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDF
                     FSYITLHLNSYPAWVPSVLPTTIRRTFTYRCWPLCPDGRSSPVIGSPRYGDNFAISKVA
                     RDMEQLLATKNLEKPLEQPENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTT
                     MVAILIGLIFLGQQLTQVGVMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARS
                     RLYRCDTYFLGKTIAELPLFLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVST
                     SFGYLISCASSSTSMALSVGPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEG
                     LLINQWADVEPGEISCTSSNTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLA
                     YLALRLRARRKE"
     gene            complement(5211..8067)
                     /gene="white"
                     /allele="+mC"
                     /label=white
     protein_bind    8566..8613
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(10387..10403)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"