LOCUS       Exported                3859 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pIGDM4RS, complete sequence.
ACCESSION   HQ845200
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Mikiewicz D, Plucienniczak A, Jagiello A, Wojtowicz-Krawiec A, 
            Chojnacka-Puchta L.
  TITLE     pIGDM4RS, the expression vector containing the tRNA ArgU gene and 
            the T7 RNA polymerase terminator sequences
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Mikiewicz D, Plucienniczak A, Jagiello A, Wojtowicz-Krawiec A, 
            Chojnacka-Puchta L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2010) Bioengineering, Institute of Biotechnology 
            and Antibiotics, Staroscinska 5, Warsaw 02516, Poland
REFERENCE   3  (bases 1 to 3859)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Expression vector pIGDM4RS"
                     /mol_type="other DNA"
                     /db_xref="taxon:1054204"
     CDS             complement(357..1217)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="confers ampicillin resistance"
                     /protein_id="AEK06413.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(357..1217)
                     /gene="bla"
                     /label=bla
     promoter        complement(1218..1322)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1354..1370
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            complement(1372..1603)
                     /gene="tRNA-Arg"
                     /label=tRNA-Arg
                     /note="codons recognized: AGA, AGG"
     tRNA            complement(1453..1529)
                     /gene="argU"
                     /product="E. coli tRNA-Arg recognizing AGA and AGG"
                     /label=argU
     regulatory      1842..1864
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     promoter        1842..1860
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1892..1914
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      1947..2096
                     /regulatory_class="terminator"
                     /note="T7 transcription terminator"
     terminator      2011..2058
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(2099..2115)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(2899..3726)
                     /label=similar to regulatory parts of ColE1-like plasmids
                     /note="similar to regulatory parts of ColE1-like plasmids"
     rep_origin      complement(3163)
                     /direction=LEFT
                     /note="OriV; similar to ColE1-like replicons"
     misc_feature    complement(3574)
                     /note="RNA I transcription start site; similar to 
                     ColE1-like replicons"
     misc_feature    complement(3679)
                     /note="RNA II transcription start site; similar to 
                     ColE1-like replicons"