LOCUS       Exported                3705 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pIGCMST, complete sequence.
ACCESSION   HQ845201
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Zaleski P, Wawrzyniak P, Sobolewska A, Mikiewicz D, 
            Wojtowicz-Krawiec A, Chojnacka-Puchta L, Zielinski M, Plucienniczak 
            G, Plucienniczak A.
  TITLE     New cloning and expression vector derived from Escherichia coli 
            plasmid pIGWZ12; a potential vector for a two-plasmid expression 
            system
  JOURNAL   Plasmid 67 (3), 264-271 (2012)
  PUBMED    22230664
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Mikiewicz D, Plucienniczak A, Jagiello A, Wojtowicz-Krawiec A, 
            Chojnacka-Puchta L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2010) Bioengineering, Institute of Biotechnology 
            and Antibiotics, Staroscinska 5, Warsaw 02516, Poland
REFERENCE   3  (bases 1 to 3705)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Expression vector pIGCMST"
                     /mol_type="other DNA"
                     /db_xref="taxon:1054203"
     misc_feature    134..961
                     /label=similar to regulatory parts of ColE1-like plasmids
                     /note="similar to regulatory parts of ColE1-like plasmids"
     misc_feature    181
                     /note="RNA II transcription start site; similar to 
                     ColE1-like replicons"
     misc_feature    286
                     /note="RNA I transcription start site; similar to 
                     ColE1-like replicons"
     rep_origin      698
                     /note="OriV; similar to ColE1-like replicons"
     promoter        1200..1302
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1303..1962
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /note="confers chloramphenicol resistance"
                     /protein_id="AEK06414.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            1303..1962
                     /gene="cat"
                     /label=cat
     regulatory      3034..3201
                     /regulatory_class="promoter"
                     /note="pms promoter"
     misc_feature    3042..3060
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     RBS             3191..3199
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3 end of the 16S rRNA (Chen et al., 1994)"
     terminator      3226..3312
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     regulatory      3229..3272
                     /regulatory_class="terminator"
                     /note="rrnB terminator T1; derived from Escherichia coli"
     regulatory      3404..3431
                     /regulatory_class="terminator"
                     /note="rrnB terminator T2; derived from Escherichia coli"
     terminator      3404..3431
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"