LOCUS       Exported                4334 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pIGALBSTP, complete sequence.
ACCESSION   HQ413773
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Chojnacka-Puchta L, Wojtowicz-Krawiec A, Jagiello A, 
            Mikiewicz-Sygula D, Szczepanska A, Plucienniczak A.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2010) Bioengineering, Institute of Biotechnology 
            and Antibiotics, Staroscinska 5, Warsaw 02-516, Poland
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Expression vector pIGALBSTP"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:927626"
     promoter        269..373
                     /gene="bla"
                     /label=AmpR promoter
     CDS             374..1234
                     /codon_start=1
                     /product="BLA"
                     /label=BLA
                     /note="beta-lactamase"
                     /protein_id="ADQ43721.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    complement(1648..1793)
                     /note="emr- putative region involved in multimer 
                     resolution; similar region found in pECO29 in INSD 
                     accession AJ001708"
     rep_origin      2997..3324
                     /label=Ori V
                     /note="Ori V"
     ncRNA           3372..3402
                     /product="RNA I"
                     /label=other
                     /ncRNA_class="other"
     ncRNA           complement(3522..3551)
                     /product="RNA II"
                     /label=other
                     /ncRNA_class="other"
     regulatory      4163..4195
                     /regulatory_class="terminator"
                     /note="ST I; stop transcription signal"
     regulatory      complement(4224..4297)
                     /regulatory_class="promoter"
                     /note="constitutive pRINT promoter; derives from weak 
                     integron In3 promoter"
     RBS             4224..4244
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(4267..4295)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     regulatory      4302..4325
                     /regulatory_class="terminator"
                     /note="St II; stop transcription signal"