LOCUS       Exported                5969 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pIE1-LUC, complete sequence.
ACCESSION   AF434923
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Zieler H, Huynh CQ.
  TITLE     Intron-dependent stimulation of marker gene expression in cultured 
            insect cells
  JOURNAL   Insect Mol. Biol. 11 (1), 87-95 (2002)
  PUBMED    11841506
REFERENCE   2  (bases 1 to 5969)
  AUTHORS   Zieler H, Huynh CQ.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2001) Medical Entomology Section, Laboratory of 
            Parasitic Diseases, National Institutes of Health, 4 Center Drive 
            MSC 0425, Bethesda, MD 20892-0425, USA
REFERENCE   3  (bases 1 to 5969)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5969
                     /organism="Expression vector pIE1-LUC"
                     /mol_type="genomic DNA"
                     /label=insect-specific
                     /note="insect-specific"
                     /db_xref="taxon:176965"
     regulatory      97..614
                     /regulatory_class="enhancer"
                     /note="Autographa californica nuclear polyhedrosis virus 
                     hr5 enhancer"
     enhancer        111..595
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     regulatory      615..1201
                     /regulatory_class="promoter"
                     /note="Autographa californica nuclear polyhedrosis virus 
                     immediate early promoter"
     CDS             1328..2980
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=firefly luciferase
                     /protein_id="AAL30778.1"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     misc_feature    3146..3251
                     /note="Heliothis virescens p63 chaperonin gene double 
                     poly-adenylation sequence"
     CDS             4168..5028
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="AAL30779.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5199..5787
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      5316..5735
                     /label=ColE1
                     /note="ColE1"