LOCUS       Exported                4327 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Gene replacement vector pIC103 DNA, complete sequence.
ACCESSION   AB257725
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Ichida H, Matsuyama T, Ryuto H, Hayashi Y, Fukunishi N, Abe T, Koba 
            T.
  TITLE     Molecular characterization of microbial mutations induced by ion 
            beam irradiation
  JOURNAL   Mutat. Res. 639 (1-2), 101-107 (2008)
  PUBMED    18068202
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Ichida H, Matsuyama T, Ryuto H, Fukunishi N, Hayashi Y, Abe T, Koba 
            T.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2006) Contact:Hiroyuki Ichida RIKEN, Nishina 
            Center for Accelerator Based Science; Hirosawa 2-1, Wako, Saitama 
            351-0198, Japan
REFERENCE   3  (bases 1 to 4327)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Gene replacement vector pIC103"
                     /focus
                     /mol_type="other DNA"
                     /db_xref="taxon:381739"
     source          1672..3387
                     /organism="Cloning vector pHP45omegaaac"
                     /mol_type="other DNA"
                     /db_xref="taxon:381740"
     primer_bind     17..33
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      53..234
                     /regulatory_class="promoter"
                     /note="Neomycin promoter of pJB866"
     CDS             235..1659
                     /codon_start=1
                     /gene="SacB"
                     /product="Levansucrase"
                     /label=SacB
                     /note="SacB gene from pYLTAC7"
                     /protein_id="BAG66059.1"
                     /translation="MNMNIKKIVKQATVLTFTTALLAGGATQAFAKENNQKAYKETYGV
                     SHITRHDMLQIPKQQQNEKYQVPQFDQSTIKNIESAKGLDVWDSWPLQNADGTVAEYNG
                     YHVVFALAGSPKDADDTSIYMFYQKVGDNSIDSWKNAGRVFKDSDKFDANDPILKDQTQ
                     EWSGSATFTSDGKIRLFYTDYSGKHYGKQSLTTAQVNVSKSDDTLKINGVEDHKTIFDG
                     DGKTYQNVQQFIDEGNYTSGDNHTLRDPHYVEDKGHKYLVFEANTGTENGYQGEESLFN
                     KAYYGGGTNFFRKESQKLQQSAKKRDAELANGALGIIELNNDYTLKKVMKPLITSNTVT
                     DEIERANVFKMNGKWYLFTDSRGSKMTIDGINSNDIYMLGYVSNSLTGPYKPLNKTGLV
                     LQMGLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFFEDKKATFAPSFLMNIKGNKT
                     SVVKNSILEQGQLTVN"
     gene            235..1659
                     /gene="SacB"
                     /label=SacB
     CDS             complement(2134..2946)
                     /codon_start=1
                     /gene="aac"
                     /product="aminoglycoside acetyltransferase"
                     /label=aac
                     /note="From pHP45omegaaac"
                     /protein_id="BAG66060.1"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEGGMRRMRCRSPVDWLSS"
     gene            complement(2134..2946)
                     /gene="aac"
                     /label=aac
     misc_feature    3342..4207
                     /label=HrcN fragment of Mesorhizobium loti MAFF303099
                     /note="HrcN fragment of Mesorhizobium loti MAFF303099"
     promoter        complement(4277..4295)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4302..4318)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"