LOCUS       Exported               10848 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle cosmid vector pHZ1358, complete sequence.
ACCESSION   AY667410
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10848)
  AUTHORS   Sun Y, Zhou X, Liu J, Bao K, Zhang G, Tu G, Kieser T, Deng Z.
  TITLE     Streptomyces nanchangensis, a producer of the insecticidal 
            polyether antibiotic nanchangmycin and the antiparasitic macrolide 
            meilingmycin, contains multiple polyketide gene clusters
  JOURNAL   Microbiology (Reading, Engl.) 148 (PT 2), 361-371 (2002)
  PUBMED    11832500
REFERENCE   2  (bases 1 to 10848)
  AUTHORS   Sun Y, Zhou X, Deng Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2004) Bio-X Life Science Research Center, Shanghai
            Jiaotong University, 1954 Huashan Road, Shanghai 200030, P. R. China
REFERENCE   3  (bases 1 to 10848)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10848
                     /organism="Shuttle cosmid vector pHZ1358"
                     /lab_host="Escherichia coli; Streptomyces"
                     /mol_type="other DNA"
                     /db_xref="taxon:291298"
     promoter        complement(4..22)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      complement(5..22)
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     promoter        137..241
                     /gene="bla"
                     /label=AmpR promoter
     CDS             242..1102
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="ampicillin resistance determinant"
                     /protein_id="AAU06201.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            242..1102
                     /gene="bla"
                     /label=bla
     rep_origin      1273..1861
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      1927..2058
                     /label=ColE1 replication origin
                     /note="ColE1 replication origin"
     polyA_signal    2257..2391
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2816..2836)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          2966..3031
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     repeat_region   3110..3145
     CDS             complement(3451..4245)
                     /codon_start=1
                     /gene="neo"
                     /product="neomycin phosphotransferase"
                     /label=neo
                     /protein_id="AAU06202.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            complement(3451..4245)
                     /gene="neo"
                     /label=neo
                     /note="no promoter"
     misc_feature    4366..4441
                     /note="pIJ101 sti region; site of second strand synthesis"
     CDS             complement(4940..6310)
                     /codon_start=1
                     /gene="rep"
                     /product="pIJ101 replication protein"
                     /label=rep
                     /note="essential for replication of plasmid pIJ101"
                     /protein_id="AAU06203.1"
                     /translation="MDPASGVIVAQTAAGTSVVLGLMRCGRIWLCPVCAATIRHKRAEE
                     ITAAVVEWIKRGGTAYLVTFTARHGHTDRLADLMDALQGTRKTPDSPRRPGAYQRLITG
                     GTWAGRRAKDGHRAADREGIRDRIGYVGMIRATEVTVGQINGWHPHIHAIVLVGGRTEG
                     ERSAKQIVATFEPTGAALDEWQGHWRSVWTAALRKVNPAFTPDDRHGVDFKRLETERDA
                     NDLAEYIAKTQDGKAPALELARADLKTATGGNVAPFELLGRIGDLTGGMTEDDAAGVGS
                     LEWNLSRWHEYERATRGRRAIEWTRYLRQMLGLDGGDTEADDLDLLLAADADGGELRAG
                     VAVTEDGWHAVTRRALDLEATRAAEGKDGNEDSAAVGERVREVLALADAADTVVVLTAG
                     EVAEAYADMLAALAQRREEATARRRREQDDDQDDDADDRQERAARHIARLASGPTSH"
     gene            complement(4940..6310)
                     /gene="rep"
                     /label=rep
     CDS             7576..8385
                     /codon_start=1
                     /gene="tsr"
                     /product="thiostrepton resistance protein"
                     /label=tsr
                     /protein_id="AAU06204.1"
                     /translation="MTELDTIANPSDPAVQRIIDVTKPSRSNIKTTLIEDVEPLMHSIA
                     AGVEFIEVYGSDSSPFPSELLDLCGRQNIPVRLIDSSIVNQLFKGERKAKTFGIARVPR
                     PARFGDIASRRGDVVVLDGVKIVGNIGAIVRTSLALGASGIILVDSDITSIADRRLQRA
                     SRGYVFSLPVVLSGREEAIAFIRDSGMQLMTLKADGDISVKELGDNPDRLALLFGSEKG
                     GPSDLFEEASSASVSIPMMSQTESLNVSVSLGIALHERIDRNLAANR"
     gene            7576..8385
                     /gene="tsr"
                     /label=tsr
     repeat_region   8526..8561
     oriT            9043..9152
                     /note="incP origin of transfer"
     CDS             9185..9556
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT recognizing protein"
                     /label=traJ
                     /note="conjugative DNA transfer"
                     /protein_id="AAU06205.1"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     gene            9185..9556
                     /gene="traJ"
                     /label=traJ
     misc_feature    10481..10776
                     /label=bacteriophage lambda COS site
                     /note="bacteriophage lambda COS site"
     promoter        10830..10848
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      10831..10848
                     /regulatory_class="promoter"
                     /note="T3 promoter"