LOCUS       Exported                5077 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHyVec5, complete sequence.
ACCESSION   DQ344484
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Steele RE, Chan TA, Bridge DM.
  TITLE     Vectors for expression of cloned genes in Hydra
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Steele RE, Chan TA, Bridge DM.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2005) Department of Biological Chemistry, 
            University of California, Irvine, 240D Medical Sciences I, Irvine, 
            CA 92697-1700, USA
REFERENCE   3  (bases 1 to 5077)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Cloning vector pHyVec5"
                     /mol_type="other DNA"
                     /db_xref="taxon:366932"
     misc_feature    1..653
                     /label=derived from pBluescript II SK+
                     /note="derived from pBluescript II SK+"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      654..1557
                     /regulatory_class="promoter"
                     /note="Hydra vulgaris actin promoter"
     CDS             1666..2379
                     /codon_start=1
                     /product="actin/red fluorescent protein fusion protein"
                     /label=actin/red fluorescent protein fusion protein
                     /protein_id="ABC69141.1"
                     /translation="MADDEVAALAAEMASSENVITEFMRFKVRMEGTVNGHEFEIEGEG
                     EGRPYEGHNTVKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFK
                     WERVMNFEDGGVATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLY
                     PRDGVLKGETHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTI
                     VEQYERTEGRHHLFL"
     misc_feature    1666..1692
                     /label=derived from Hydra vulgaris actin gene
                     /note="derived from Hydra vulgaris actin gene"
     misc_feature    1702..2376
                     /label=red fluorescent protein
                     /note="red fluorescent protein"
     misc_feature    2380..2875
                     /note="3 flanking sequence from Hydra vulgaris actin gene"
     regulatory      2511..2516
                     /regulatory_class="polyA_signal_sequence"
     misc_feature    2875..5077
                     /label=derived from pBluescript II SK+
                     /note="derived from pBluescript II SK+"
     promoter        complement(2889..2907)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2928..2944)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2952..2968
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2976..3006)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3021..3042
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3330..3918)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4089..4949)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="ampicillin resistance protein"
                     /protein_id="ABC69142.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4950..5054)
                     /gene="bla"
                     /label=AmpR promoter