LOCUS       Exported                5112 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHyVec3, complete sequence.
ACCESSION   AY589050
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Steele RE, Wiley CA, Chan TA.
  TITLE     Vectors for expression of cloned genes in Hydra
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5112)
  AUTHORS   Steele RE, Wiley CA, Chan TA.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2004) Dept. of Biological Chemistry, University of
            California, Irvine, 240D Medical Sciences I, Irvine, CA 92697-1700, 
            USA
REFERENCE   3  (bases 1 to 5112)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Cloning vector pHyVec3"
                     /mol_type="other DNA"
                     /db_xref="taxon:272941"
     misc_feature    1..653
                     /label=derived from pBluescript II SK+
                     /note="derived from pBluescript II SK+"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      654..1557
                     /regulatory_class="promoter"
                     /note="Hydra vulgaris actin promoter"
     misc_feature    1666..1668
                     /label=Hydra vulgaris actin start codon
                     /note="Hydra vulgaris actin start codon"
     CDS             1698..2414
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=green fluorescent protein
                     /protein_id="AAT02644.1"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    2415..2910
                     /label=3 flanking sequence from Hydra vulgaris actin
                     /note="3 flanking sequence from Hydra vulgaris actin"
     regulatory      2546..2551
                     /regulatory_class="polyA_signal_sequence"
     misc_feature    2911..5112
                     /label=derived from pBluescript II SK+
                     /note="derived from pBluescript II SK+"
     promoter        complement(2924..2942)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2963..2979)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2987..3003
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3011..3041)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3056..3077
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3365..3953)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4124..4984)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="ampicillin resistance protein"
                     /protein_id="AAT02645.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4985..5089)
                     /gene="bla"
                     /label=AmpR promoter