LOCUS       Exported                4368 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHyVec2, complete sequence.
ACCESSION   AY574915
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Steele RE, Wiley CA, Chan TA.
  TITLE     Vectors for expression of cloned genes in Hydra
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Steele RE, Wiley CA, Chan TA.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2004) Dept. of Biological Chemistry, University of
            California, Irvine, 240D Medical Sciences I, Irvine, CA 92697-1700, 
            USA
REFERENCE   3  (bases 1 to 4368)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Cloning vector pHyVec2"
                     /mol_type="other DNA"
                     /db_xref="taxon:270493"
     misc_feature    1..653
                     /label=pBluescript II SK+ vector sequence
                     /note="pBluescript II SK+ vector sequence"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      654..1557
                     /regulatory_class="promoter"
                     /note="Hydra actin promoter"
     misc_feature    1666..1678
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    1666..1668
                     /label=Hydra actin start codon
                     /note="Hydra actin start codon"
     misc_feature    1679..2166
                     /label=Hydra actin 3 flanking sequence
                     /note="Hydra actin 3 flanking sequence"
     regulatory      1802..1807
                     /regulatory_class="polyA_signal_sequence"
     misc_feature    2167..4368
                     /label=pBluescript II SK+ vector sequence
                     /note="pBluescript II SK+ vector sequence"
     promoter        complement(2180..2198)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2219..2235)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2243..2259
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2267..2297)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2312..2333
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(2621..3209)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3380..4240)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="ampicillin resistance protein"
                     /protein_id="AAS87367.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4241..4345)
                     /gene="bla"
                     /label=AmpR promoter