LOCUS       Exported                5373 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pHyVec16, complete sequence.
ACCESSION   KP145000
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Steele RE, Dana CE.
  TITLE     Vectors for expression of cloned genes in hydra
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Steele RE, Dana CE.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2014) Department of Biological Chemistry, 
            University of California, Irvine, D240 Medical Sciences I, Irvine, 
            CA 92697-1700, USA
REFERENCE   3  (bases 1 to 5373)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Expression vector pHyVec16"
                     /mol_type="other DNA"
                     /db_xref="taxon:1629123"
     protein_bind    400..499
                     /label=phi-C31 attP binding site
                     /bound_moiety="phi-C31 attP"
     primer_bind     896..912
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        922..940
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      950..1853
                     /regulatory_class="promoter"
                     /note="hydra actin promoter"
     CDS             1962..2675
                     /codon_start=1
                     /product="actin-DsRed2 fusion protein"
                     /label=actin-DsRed2 fusion protein
                     /note="amino terminus of hydra actin fused to DsRed2"
                     /protein_id="AJY78445.1"
                     /translation="MADDEVAALAAEMASSENVITEFMRFKVRMEGTVNGHEFEIEGEG
                     EGRPYEGHNTVKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFK
                     WERVMNFEDGGVATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLY
                     PRDGVLKGETHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTI
                     VEQYERTEGRHHLFL"
     misc_feature    2676..3171
                     /label=hydra actin 3 flanking sequence
                     /note="hydra actin 3 flanking sequence"
     regulatory      2807..2812
                     /regulatory_class="polyA_signal_sequence"
     promoter        complement(3185..3203)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3224..3240)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3248..3264
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3272..3302)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3317..3338
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3626..4214)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4385..5245)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="AJY78446.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5246..5350)
                     /gene="bla"
                     /label=AmpR promoter