LOCUS       Exported                5153 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pHyVec14, complete sequence.
ACCESSION   KP119774
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Steele RE, Dana CE.
  TITLE     Vectors for expression of cloned genes in Hydra
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Steele RE, Dana CE.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2014) Department of Biological Chemistry, 
            University of California, Irvine, D240 Medical Sciences I, Irvine, 
            CA 92697-1700, USA
REFERENCE   3  (bases 1 to 5153)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Expression vector pHyVec14"
                     /mol_type="other DNA"
                     /db_xref="taxon:1629606"
     misc_feature    304..373
                     /label=multiple cloning site
                     /note="multiple cloning site"
     primer_bind     676..692
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        702..720
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      730..1633
                     /regulatory_class="promoter"
                     /note="derived from Hydra actin gene"
     CDS             1742..2455
                     /codon_start=1
                     /product="actin-DsRed2 fusion protein"
                     /label=actin-DsRed2 fusion protein
                     /note="includes the amino terminal sequence of Hydra actin 
                     and the sequence of DsRed2"
                     /protein_id="AJT59681.1"
                     /translation="MADDEVAALAAEMASSENVITEFMRFKVRMEGTVNGHEFEIEGEG
                     EGRPYEGHNTVKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFK
                     WERVMNFEDGGVATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLY
                     PRDGVLKGETHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTI
                     VEQYERTEGRHHLFL"
     misc_feature    2456..2951
                     /label=3 flanking sequence from Hydra actin
                     /note="3 flanking sequence from Hydra actin"
     regulatory      2587..2592
                     /regulatory_class="polyA_signal_sequence"
     promoter        complement(2965..2983)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3004..3020)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3028..3044
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3052..3082)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3097..3118
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3406..3994)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4165..5025)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="AJT59680.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5026..5130)
                     /gene="bla"
                     /label=AmpR promoter