LOCUS       Exported                5204 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHyVec13, complete sequence.
ACCESSION   JN982438
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Steele RE, Dana CE.
  TITLE     Vectors for expression of cloned gene in Hydra
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5204)
  AUTHORS   Steele RE, Dana CE.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2011) Department of Biological Chemistry, 
            University of California, Irvine, D240 Medical Sciences I, Irvine, 
            CA 92697-1700, USA
REFERENCE   3  (bases 1 to 5204)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Cloning vector pHyVec13"
                     /mol_type="other DNA"
                     /db_xref="taxon:1156763"
     misc_feature    1..729
                     /label=derived from pBluescript II SK+
                     /note="derived from pBluescript II SK+"
     misc_feature    304..373
                     /label=multiple cloning site
                     /note="multiple cloning site"
     primer_bind     676..692
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        702..720
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      730..1633
                     /regulatory_class="promoter"
                     /note="derived from Hydra vulgaris actin"
     CDS             1742..2506
                     /codon_start=1
                     /product="actin/green fluorescent protein fusion protein"
                     /label=actin/green fluorescent protein fusion protein
                     /protein_id="AFC87614.1"
                     /translation="MADDEVAALAAAPVEKMSKGEELFTGVVPILVELDGDVNGHKFSV
                     SGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAM
                     PEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNS
                     HNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSA
                     LSKDPNEKRDHMVLLEFVTAAGITHGMDELYK"
     misc_feature    1742..1768
                     /label=derived from Hydra vulgaris actin
                     /note="derived from Hydra vulgaris actin"
     CDS             1790..2506
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    1790..2503
                     /label=derived from green fluorescent protein
                     /note="derived from green fluorescent protein"
     misc_feature    2507..3002
                     /label=3 flanking sequence from Hydra vulgaris actin
                     /note="3 flanking sequence from Hydra vulgaris actin"
     regulatory      2638..2643
                     /regulatory_class="polyA_signal_sequence"
     misc_feature    3003..5204
                     /label=derived from pBluescript II SK+
                     /note="derived from pBluescript II SK+"
     promoter        complement(3016..3034)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3055..3071)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3079..3095
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3103..3133)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3148..3169
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3457..4045)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4216..5076)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="AFC87615.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5077..5181)
                     /gene="bla"
                     /label=AmpR promoter