LOCUS       Exported                5525 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHyVec11, complete sequence.
ACCESSION   EU183365
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Dana CE, Glauber KM, Chan TA, Bridge DM, Steele RE.
  TITLE     Incorporation of a horizontally transferred gene into an operon 
            during cnidarian evolution
  JOURNAL   PLoS ONE 7 (2), E31643 (2012)
  PUBMED    22328943
REFERENCE   2  (bases 1 to 5525)
  AUTHORS   Steele R, Chan T, Dana C.
  TITLE     Vectors for expression of cloned genes in Hydra
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5525)
  AUTHORS   Steele R, Chan T, Dana C.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2007) Department of Biological Chemistry, 
            University of California, Irvine, 240D Medical Sciences I, Irvine, 
            CA 92697-1700, USA
REFERENCE   4  (bases 1 to 5525)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Cloning vector pHyVec11"
                     /mol_type="other DNA"
                     /db_xref="taxon:477927"
     misc_feature    1..653
                     /label=derived from pBluescript II SK+
                     /note="derived from pBluescript II SK+"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      654..1557
                     /regulatory_class="promoter"
                     /note="Hydra vulgaris actin"
     misc_feature    1665..1691
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    1696..2131
                     /note="intergenic region between Hydra replication factor C
                     large subunit gene and flp gene"
     misc_feature    2112..2113
                     /note="splice acceptor dinucleotide for trans-spliced 
                     leader"
     CDS             2132..2827
                     /codon_start=1
                     /gene="DsRed2"
                     /product="red fluorescent protein"
                     /label=DsRed2
                     /protein_id="ABW38021.1"
                     /translation="MADAAEMASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYE
                     GHNTVKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN
                     FEDGGVATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVL
                     KGETHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYER
                     TEGRHHLFL"
     gene            2132..2827
                     /gene="DsRed2"
                     /label=DsRed2
     misc_feature    2828..3323
                     /note="3 flanking sequence from Hydra vulgaris actin gene"
     regulatory      2959..2964
                     /regulatory_class="polyA_signal_sequence"
     misc_feature    3324..5525
                     /label=derived from pBluescript II SK+
                     /note="derived from pBluescript II SK+"
     promoter        complement(3337..3355)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3376..3392)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3400..3416
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3424..3454)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3469..3490
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3778..4366)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4537..5397)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="ampicillin resistance protein"
                     /protein_id="ABW38022.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5398..5502)
                     /gene="bla"
                     /label=AmpR promoter