LOCUS       Exported                5387 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pHY320PLK DNA, complete sequence.
ACCESSION   D00946
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Ishiwa H, Shibahara H.
  TITLE     New shuttle vectors for Escherichia coli and Bacillus subtilis. III.
            Nucleotide sequence analysis of tetracycline resistance gene of 
            pAMalpha1 and ori-177
  JOURNAL   Jpn. J. Genet. 60, 485-498 (1985)
REFERENCE   2  (bases 1 to 5387)
  AUTHORS   Ishiwa H, Shibahara-Sone H.
  TITLE     New shuttle vectors for Escherichia coli and Bacillus subtilis. IV. 
            The nucleotide sequence of pHY300PLK and some properties in relation
            to transformation
  JOURNAL   Jpn. J. Genet. 61, 515-528 (1986)
REFERENCE   3  (bases 1 to 5387)
  AUTHORS   Ishiwa H, Shibahara-Sone H, Shimizu-Kadota M.
  TITLE     New shuttle vectors for Escherichia coli and Bacillus subtilis. V
  JOURNAL   Unpublished
REFERENCE   4  (bases 1 to 5387)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Shuttle vector pHY320PLK"
                     /mol_type="other DNA"
                     /db_xref="taxon:77677"
     misc_feature    1..51
                     /label=polylinker
                     /note="polylinker"
     rep_origin      102..519
                     /label=BA3 minus origin
                     /note="BA3 minus origin"
     CDS             complement(616..1992)
                     /codon_start=1
                     /product="tetracycline resistance protein"
                     /label=tetracycline resistance protein
                     /note="gtg is a start codon"
                     /protein_id="BAA20952.1"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFS
                     LLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIA
                     HYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYS
                     ISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPY
                     MMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTA
                     SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGT
                     GIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQRDF
                     "
     regulatory      complement(2000..2004)
                     /regulatory_class="ribosome_binding_site"
     regulatory      complement(2125..2130)
                     /regulatory_class="minus_10_signal"
     regulatory      complement(2148..2153)
                     /regulatory_class="minus_35_signal"
     CDS             complement(2199..3203)
                     /codon_start=1
                     /gene="repB"
                     /product="RepB replication protein"
                     /label=repB
                     /note="from Enterococcus faecalis plasmid pAM-alpha-1"
                     /db_xref="GI:22652809"
                     /protein_id="AAN03827.1"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     CDS             complement(2199..3182)
                     /codon_start=1
                     /product="Rep-alpha-1"
                     /label=Rep-alpha-1
                     /note="replication protein"
                     /protein_id="BAA00794.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHIL
                     EFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKVVA
                     EVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMRATE
                     VTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKVQMIR
                     PKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYGGLLKE
                     IHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     regulatory      complement(3197..3201)
                     /regulatory_class="ribosome_binding_site"
     variation       3278..3279
                     /replace="tcc"
                     /citation="[2]"
     regulatory      complement(3285..3291)
                     /regulatory_class="minus_10_signal"
     regulatory      complement(3308..3313)
                     /regulatory_class="minus_35_signal"
     regulatory      complement(3336..3342)
                     /regulatory_class="minus_10_signal"
     regulatory      complement(3357..3364)
                     /regulatory_class="minus_35_signal"
     rep_origin      complement(3361..3362)
                     /direction=LEFT
                     /note="replication origin in Gram-positive bacteria (nick 
                     site)"
     variation       3596
                     /citation="[2]"
     variation       3699
                     /replace="c"
                     /citation="[2]"
     promoter        3700..3804
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      3735..3740
                     /regulatory_class="minus_35_signal"
     regulatory      3758..3763
                     /regulatory_class="minus_10_signal"
     regulatory      3794..3801
                     /regulatory_class="ribosome_binding_site"
     CDS             3805..4665
                     /codon_start=1
                     /product="ampicillin resistance protein"
                     /label=ampicillin resistance protein
                     /protein_id="BAA00795.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     variation       4350
                     /replace="a"
                     /citation="[2]"
     rep_origin      4830..5374
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     misc_feature    4866..5376
                     /label=RNA primer
                     /note="RNA primer"
     variation       4949
                     /replace="c"
                     /citation="[2]"
     variation       5378
                     /citation="[2]"