LOCUS       Exported                6185 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  HaloTag CMV-neo vector pHTC, complete sequence.
ACCESSION   JF920305
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6185)
  AUTHORS   Hartnett J, Slater M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2011) Promega Corporation, 2800 Woods Hollow Road,
            Madison, WI 53711, USA
REFERENCE   2  (bases 1 to 6185)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6185
                     /organism="HaloTag CMV-neo vector pHTC"
                     /mol_type="other DNA"
                     /db_xref="taxon:1093973"
     regulatory      1..742
                     /regulatory_class="promoter"
                     /note="CMV immediate/early promoter"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          857..989
                     /note="chimeric intron"
     regulatory      1033..1052
                     /regulatory_class="promoter"
                     /note="T7 RNA polymerase promoter"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1052..1129
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    1124..1168
                     /label=HaloTag linker C terminus
                     /note="HaloTag linker C terminus"
     misc_feature    1139..1159
                     /label=TEV protease recognition site
                     /note="TEV protease recognition site"
     CDS             1139..1159
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     misc_feature    1169..2059
                     /label=HaloTag C-terminal tag
                     /note="HaloTag C-terminal tag"
     CDS             1175..2056
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEIS"
     regulatory      2177..2398
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 late polyadenylation signal"
     polyA_signal    2186..2307
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      2497..2915
                     /regulatory_class="promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        2552..2909
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2760..2895
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      2813..2878
                     /label=SV40 minimum origin of replication
                     /note="SV40 minimum origin of replication"
     CDS             2960..3754
                     /codon_start=1
                     /gene="neoR"
                     /product="neomycin phosphotransferase"
                     /label=neoR
                     /protein_id="AEQ28653.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            2960..3754
                     /gene="neoR"
                     /label=neoR
                     /note="antibiotic resistance protein"
     regulatory      3818..3866
                     /regulatory_class="polyA_signal_sequence"
                     /note="synthetic polyadenylation signal"
     polyA_signal    3818..3866
                     /note="synthetic polyadenylation signal"
     promoter        4022..4126
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4127..4987
                     /codon_start=1
                     /gene="ampR"
                     /product="beta-lactamase"
                     /label=ampR
                     /protein_id="AEQ28652.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            4127..4987
                     /gene="ampR"
                     /label=ampR
     rep_origin      5142..5178
                     /label=ColE1-derived origin of replication
                     /note="ColE1-derived origin of replication"
     rep_origin      complement(5145..5733)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5849..6132
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"