LOCUS       Exported                7398 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHT-gfpBteAAV, complete sequence.
ACCESSION   MF673728
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Ben Rejeb S, Lereclus D, Slamti L.
  TITLE     Analysis of abrB Expression during the Infectious Cycle of Bacillus 
            thuringiensis Reveals Population Heterogeneity
  JOURNAL   Front Microbiol 8, 2471 (2017)
  PUBMED    29312181
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   Ben Rejeb S, Slamti L.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2017) MICA-Institut Micalis, INRA, Domaine de 
            Vilvert, Jouy-en-Josas 78350, France
REFERENCE   3  (bases 1 to 7398)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Cloning vector pHT-gfpBteAAV"
                     /mol_type="other DNA"
                     /db_xref="taxon:2073114"
     RBS             49..57
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3 end of the 16S rRNA (Chen et al., 1994)"
     CDS             64..855
                     /codon_start=1
                     /product="GFPBteAAV"
                     /label=GFPBteAAV
                     /note="codon-adapted to Bacillus thuringiensis; includes a 
                     3-encoded destabilizing tag"
                     /protein_id="AUR53415.1"
                     /translation="MNGIEIFAMSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDA
                     TNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQER
                     TISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITAD
                     KQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEK
                     RDHMVLLEFVTAAGITHGMDELYKGSHHHHHGKQNNLSAAV"
     primer_bind     complement(887..903)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    911..927
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(935..965)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    980..1001
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1289..1877)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2048..2908)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7344..7360
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"