LOCUS       Exported                4344 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHSP-MCS-mGFP, complete sequence.
ACCESSION   KP893621
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Saidi Y, Moody LA, Coates JC.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAR-2015) Department of Biosciences, University of 
            Birmingham, Edgbaston, Birmingham B15 2TT, UK
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Cloning vector pHSP-MCS-mGFP"
                     /mol_type="other DNA"
                     /db_xref="taxon:1643433"
     regulatory      21..521
                     /regulatory_class="promoter"
                     /note="Gmhsp17.5"
     misc_feature    522..557
                     /label=MCS
                     /note="MCS"
     CDS             558..1274
                     /codon_start=1
                     /gene="mGFP5"
                     /product="mGFP5"
                     /label=mGFP5
                     /protein_id="AKE44004.1"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHHLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     gene            558..1274
                     /gene="mGFP5"
                     /label=mGFP5
     regulatory      1281..1475
                     /regulatory_class="terminator"
                     /note="35S Ter"
     promoter        complement(1490..1508)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1518..1534)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(1675..2130)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2157..2261
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2262..3122
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3293..3881
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4169..4190
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4205..4235
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4243..4259
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4267..4283
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4304..4322
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"