LOCUS       Exported                6949 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHSG415s DNA, complete sequence.
ACCESSION   AB095955
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6949)
  AUTHORS   Hashimoto-Gotoh T, Franklin FC, Nordheim A, Timmis KN.
  TITLE     Specific-purpose plasmid cloning vectors. I. Low copy number, 
            temperature-sensitive, mobilization-defective pSC101-derived 
            containment vectors
  JOURNAL   Gene 16 (1-3), 227-235 (1981)
  PUBMED    6282694
REFERENCE   2  (bases 1 to 6949)
  AUTHORS   Hashimoto-Gotoh T.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2002) Tam Hashimoto-Gotoh, Kyoto Pref. Univ. of 
            Med., RINDG, Dept. of Biochem. and Mol. Genet.; 
            Kawaramachi-Hirokoji, Kamigyo-Ku, Kyoto, Kyoto 602-8566, Japan 
            (E-mail:e54093@sakura.kudpc.kyoto-u.ac.jp, Tel:81-75-251-5798, 
            Fax:81-75-251-5799)
REFERENCE   3  (bases 1 to 6949)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6949
                     /organism="Cloning vector pHSG415s"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:213659"
     promoter        complement(217..319)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      817..1039
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             1087..2037
                     /codon_start=1
                     /gene="repAtr"
                     /product="pSC101 DNA replication initiator temperature 
                     sensitive"
                     /label=repAtr
                     /protein_id="BAC23911.1"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     gene            1087..2037
                     /gene="repAtr"
                     /label=repAtr
     misc_difference 1253
                     /gene="repAtr"
                     /replace="c"
                     /note="temperature sensitive mutation: wild type is 
                     reported in acc#: AB095956"
     CDS             complement(3500..4360)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="BAC23912.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3500..4360)
                     /gene="bla"
                     /label=bla
     promoter        complement(4361..4465)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(5060..5875)
                     /codon_start=1
                     /gene="agp"
                     /product="aminoglucoside phosphotransferase"
                     /label=agp
                     /protein_id="BAC23913.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(5060..5875)
                     /gene="agp"
                     /label=agp
     CDS             complement(6506..216)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyl transferase"
                     /label=cat
                     /protein_id="BAC23914.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            complement(6506..216)
                     /gene="cat"
                     /label=cat