LOCUS       Exported                7207 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Lentiviral transfer vector pHsCXW, complete sequence.
ACCESSION   AY468486
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7207)
  AUTHORS   Leander Johansen J, Dago L, Tornoe J, Rosenblad C, Kusk P.
  TITLE     A new versatile and compact lentiviral vector
  JOURNAL   Mol. Biotechnol. 29 (1), 47-56 (2005)
  PUBMED    15668519
REFERENCE   2  (bases 1 to 7207)
  AUTHORS   Johansen J, Tornoe J, Rosenblad C, Dago L, Kusk P.
  TITLE     Improved lentiviral transfer vector
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7207)
  AUTHORS   Kusk P, Johansen J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2003) NsGene A/S, Baltorpvej 154, Ballerup 
            DK-2750, Denmark
REFERENCE   4  (bases 1 to 7207)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7207
                     /organism="Lentiviral transfer vector pHsCXW"
                     /mol_type="other DNA"
                     /db_xref="taxon:256321"
     repeat_region   1..634
                     /label=5 HIV-1 LTR
                     /note="5 HIV-1 LTR"
     LTR             1..634
                     /label=3 LTR
                     /note="3 long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     regulatory      686..823
                     /regulatory_class="other"
                     /note="packaging signal; psi"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     regulatory      1310..1514
                     /regulatory_class="other"
                     /note="Rev responsive element; RRE"
     regulatory      2031..2547
                     /regulatory_class="promoter"
                     /note="CMV promoter"
     enhancer        2040..2343
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2344..2547
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    2618..2671
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    complement(2674..2693)
                     /label=T7 recognition/binding site
                     /note="T7 recognition/binding site"
     promoter        complement(2675..2693)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2711..3315
                     /note="Woodchuck post-regulatory element; WPRE"
     misc_feature    2721..3309
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(3192..3203)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     repeat_region   3512..3746
                     /note="3 LTR; self-inactivating 3 LTR version; distal 
                     promoter of U3 deleted"
     LTR             3514..3746
                     /label=3 LTR (Delta-U3)
                     /note="self-inactivating 3 long terminal repeat (LTR) from
                     HIV-1"
     rep_origin      4719..5392
     rep_origin      complement(4778..5366)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5537..6397)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="ampicillin resistance gene"
                     /protein_id="AAR24091.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(5537..6397)
                     /gene="bla"
                     /label=bla
     promoter        complement(6398..6502)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6906..7040
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"