LOCUS       Exported                4304 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector phRG(R2.2), complete sequence.
ACCESSION   AY487824
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Almond BD, Chauvin F, Kenefick KB.
  TITLE     Rapid Response (TM) Reporter Vectors Technical Manual, TM242
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Almond BD, Chauvin F, Kenefick KB.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2003) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 4304)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Reporter vector phRG(R2.2)"
                     /mol_type="other DNA"
                     /db_xref="taxon:257729"
     misc_feature    1..58
                     /label=multiple cloning region
                     /note="multiple cloning region"
     regulatory      82..91
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             88..1200
                     /codon_start=1
                     /product="hRlucCP reporter protein"
                     /label=hRlucCP reporter protein
                     /protein_id="AAR29597.1"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQNSACKNWFSSLSHFVIHLNSHGFPPEVEE
                     QAAGTLPMSCAQESGMDRHPAACASARINV"
     CDS             88..1020
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             1027..1074
                     /codon_start=1
                     /product="non-ORF yeast peptide conferring 
                     ubiquitin-dependent degradation"
                     /label=hCL1
                     /note="human codon-optimized"
                     /translation="ACKNWFSSLSHFVIHL"
     CDS             1078..1197
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    1258..1479
                     /label=SV40 late poly (A) region
                     /note="SV40 late poly (A) region"
     polyA_signal    1267..1388
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     1547..1566
                     /label=reporter vector primer 4 (RVPrimer4)
                     /note="reporter vector primer 4 (RVPrimer4)"
     rep_origin      1804
                     /label=ColE1-derived
                     /note="ColE1-derived"
     rep_origin      complement(1807..2395)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2566..3426)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="AmpR"
                     /protein_id="AAR29598.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3427..3531)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3558..4013
                     /label=f1
                     /note="f1"
     misc_feature    4144..4297
                     /label=upstream synthetic poly (A) region
                     /note="upstream synthetic poly (A) region"
     polyA_signal    4144..4192
                     /note="synthetic polyadenylation signal"
     misc_feature    4206..4297
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4246..4265
                     /label=reporter vector primer 3 (RVPrimer3)
                     /note="reporter vector primer 3 (RVPrimer3)"