LOCUS       Exported                4232 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector phRG(R2.1), complete sequence.
ACCESSION   AY487823
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Almond BD, Chauvin F, Kenefick KB.
  TITLE     Rapid Response (TM) Reporter Vectors Technical Manual, TM242
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Almond BD, Chauvin F, Kenefick KB.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2003) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 4232)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Reporter vector phRG(R2.1)"
                     /mol_type="other DNA"
                     /db_xref="taxon:257728"
     misc_feature    1..58
                     /label=multiple cloning region
                     /note="multiple cloning region"
     regulatory      82..91
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             88..1146
                     /codon_start=1
                     /product="hRlucP reporter protein"
                     /label=hRlucP reporter protein
                     /protein_id="AAR29595.1"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQNSHGFPPEVEEQAAGTLPMSCAQESGMDR
                     HPAACASARINV"
     CDS             88..1020
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             1024..1143
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    1186..1407
                     /label=SV40 late poly (A) region
                     /note="SV40 late poly (A) region"
     polyA_signal    1195..1316
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     1475..1494
                     /label=reporter vector primer 4 (RVPrimer4)
                     /note="reporter vector primer 4 (RVPrimer4)"
     rep_origin      1732
                     /label=ColE1-derived
                     /note="ColE1-derived"
     rep_origin      complement(1735..2323)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2494..3354)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="AmpR"
                     /protein_id="AAR29596.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3355..3459)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3486..3941
                     /label=f1
                     /note="f1"
     misc_feature    4072..4225
                     /label=upstream synthetic poly (A) region
                     /note="upstream synthetic poly (A) region"
     polyA_signal    4072..4120
                     /note="synthetic polyadenylation signal"
     misc_feature    4134..4225
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4174..4193
                     /label=reporter vector primer 3 (RVPrimer3)
                     /note="reporter vector primer 3 (RVPrimer3)"