LOCUS       Exported               10725 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHR307a, complete sequence.
ACCESSION   DQ012948
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10725)
  AUTHORS   Mastick GS, McKay R, Oligino T, Donovan K, Lopez AJ.
  TITLE     Identification of target genes regulated by homeotic proteins in 
            Drosophila melanogaster through genetic selection of Ultrabithorax 
            protein-binding sites in yeast
  JOURNAL   Genetics 139 (1), 349-363 (1995)
  PUBMED    7705635
REFERENCE   2  (bases 1 to 10725)
  AUTHORS   Hollenbach AD, Ng S-S., Flynn AP.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2005) Genetics, Louisiana State University Health 
            Sciences Center, 533 Bolivar Street, New Orleans, LA 70119, USA
REFERENCE   3  (bases 1 to 10725)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10725
                     /organism="Cloning vector pHR307a"
                     /mol_type="other DNA"
                     /db_xref="taxon:328375"
     misc_feature    1..50
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      51..360
                     /regulatory_class="promoter"
                     /note="GAL1 minimal promoter"
     gene            366..1944
                     /gene="HIS3"
                     /label=HIS3
     CDS             366..1028
                     /codon_start=1
                     /gene="HIS3"
                     /product="HIS3"
                     /label=HIS3
                     /protein_id="AAY40259.1"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     3UTR           1029..1944
                     /gene="HIS3"
     promoter        2738..2953
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     gene            complement(3160..4630)
                     /gene="TRP1"
                     /label=TRP1
     3UTR           complement(3160..3848)
                     /gene="TRP1"
     rep_origin      3173..4009
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     CDS             complement(3896..4522)
                     /codon_start=1
                     /gene="TRP1"
                     /product="TRP1"
                     /label=TRP1
                     /protein_id="AAY40260.1"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGGRQMV"
     5UTR           complement(4523..4630)
                     /gene="TRP1"
     promoter        complement(4523..4624)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     misc_feature    6017..7670
                     /label=CEN4 sequence
                     /note="CEN4 sequence"
     misc_feature    6576..6868
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     misc_feature    7695..8435
                     /label=ARS1 sequence
                     /note="ARS1 sequence"
     misc_feature    8575..8715
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8901..9489)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      9314..9464
                     /label=ColE1 origin of replication
                     /note="ColE1 origin of replication"
     CDS             complement(9660..10520)
                     /codon_start=1
                     /product="Amp"
                     /label=Amp
                     /protein_id="AAY40261.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10521..10625)
                     /gene="bla"
                     /label=AmpR promoter