LOCUS       Exported               11686 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHR-CMVLacZ, complete sequence.
ACCESSION   AF105229
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11686)
  AUTHORS   Naldini L, Blomer U, Gallay P, Ory D, Mulligan R, Gage FH, Verma IM,
            Trono D.
  TITLE     In vivo gene delivery and stable transduction of nondividing cells 
            by a lentiviral vector
  JOURNAL   Science 272 (5259), 263-267 (1996)
  PUBMED    8602510
REFERENCE   2  (bases 1 to 11686)
  AUTHORS   Zufferey R, Dull T, Mandel RJ, Bukovsky A, Quiroz D, Naldini L, 
            Trono D.
  TITLE     Self-inactivating lentivirus vector for safe and efficient in vivo 
            gene delivery
  JOURNAL   J. Virol. 72 (12), 9873-9880 (1998)
  PUBMED    9811723
REFERENCE   3  (bases 1 to 11686)
  AUTHORS   Cherepanov P, Claeys A, Trono D, Debyser Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-1998) AIDS Laboratory, Rega Institute for Medical 
            Research, Minderbroedersstr. 10, Leuven 3000, Belgium
REFERENCE   4  (bases 1 to 11686)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11686
                     /organism="Cloning vector pHR-CMVLacZ"
                     /mol_type="genomic DNA"
                     /label=HIV1-derived lentiviral vector
                     /note="HIV1-derived lentiviral vector"
                     /db_xref="taxon:85619"
     repeat_region   1..636
     LTR             3..636
                     /label=5 LTR
                     /note="5 long terminal repeat (LTR) from HIV-1"
     promoter        complement(661..679)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        1467..1571
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1572..2432
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="AAC97515.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            1572..2432
                     /gene="bla"
                     /label=bla
                     /note="ampicillin resistance gene"
     rep_origin      2603..3191
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      3197
                     /label=pUC replication origin
                     /note="pUC replication origin"
     promoter        3437..3766
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     regulatory      3486..3741
                     /regulatory_class="promoter"
                     /note="SV40 promoter-enhancer"
     rep_origin      3617..3752
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    3966..4423
                     /gene="gpt"
                     /note="gpt open reading frame disrupted by a frame shift 
                     resulting in loss of the KpnI site in the gpt gene"
     gene            3966..4423
                     /gene="gpt"
                     /label=gpt
                     /note="xanthine guanine phosphoribosyl transferase"
     misc_feature    4825..5674
                     /label=SV40 polyadenylation signal and intron
                     /note="SV40 polyadenylation signal and intron"
     intron          4900..4965
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5095..5115
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5540..5674
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   5843..6476
     LTR             5843..6476
                     /label=3 LTR
                     /note="3 long terminal repeat (LTR) from HIV-1"
     misc_feature    6523..6648
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6528..6665
                     /label=HIV-1 genomic RNA packaging signal
                     /note="HIV-1 genomic RNA packaging signal"
     misc_feature    6585..6588
                     /label=5-splice site
                     /note="5-splice site"
     misc_feature    6632..6634
                     /label=gag gene start site
                     /note="gag gene start site"
     misc_feature    6674..6675
                     /note="gag ORF disrupted by a frame shift; ClaI site 
                     filled-in"
     misc_feature    7141..7374
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7152..7356
                     /note="Rev responsive element; RRE"
     misc_feature    7748..7750
                     /label=3-splice site
                     /note="3-splice site"
     regulatory      7873..8389
                     /regulatory_class="promoter"
                     /note="hCMV immediate early promoter"
     enhancer        7882..8185
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8186..8389
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             8506..11565
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /protein_id="AAC97516.1"
                     /translation="MLDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQ
                     QLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYHAPIYTNVTYPIT
                     VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRL
                     PSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFH
                     VATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGY
                     ADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLL
                     NGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCD
                     RYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNES
                     GHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIK
                     KWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDE
                     NGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVT
                     SEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTV
                     RVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQ
                     FNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHY
                     QAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASD
                     TPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSE
                     NGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGI
                     GGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            8506..11565
                     /gene="lacZ"
                     /label=lacZ
                     /note="Escherichia coli beta-galactosidase reporter gene; 
                     multiple cloning site for BamHI, EcoRI, KpnI, XhoI"
     CDS             8518..11565
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYHAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"