LOCUS       Exported                4079 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pHQEff-6, complete sequence.
ACCESSION   KJ184338
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Ma JZ, Ma YL, Huang F.
  TITLE     An efficient plant transient expression vector system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Ma JZ, Ma YL, Huang F.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2014) School of Life Science and Engineering, 
            LanZhou University of Technology, No.287 Langongping Road, 
            QiliheDistrict, Lanzhou 730050, China
REFERENCE   3  (bases 1 to 4079)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Expression vector pHQEff-6"
                     /mol_type="other DNA"
                     /db_xref="taxon:1483520"
     rep_origin      complement(183..771)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(942..1802)
                     /codon_start=1
                     /product="ampicillin resistance protein"
                     /label=ampicillin resistance protein
                     /protein_id="AHV90402.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1803..1907)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2381..2397
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2414..2944
                     /regulatory_class="promoter"
                     /note="35S promoter from CaMV"
     promoter        2597..2942
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    2971..3408
                     /label=GAL4 DNA binding domain
                     /note="GAL4 DNA binding domain"
     CDS             2971..3408
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     promoter        3419..3437
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3454..3486
                     /label=c-Myc epitope tag
                     /note="c-Myc epitope tag"
     CDS             3457..3486
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    3511..3579
                     /note="partial Arabidopsis thaliana ABI5-family 
                     transcription factor"
     terminator      3586..3838
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      3605..3841
                     /regulatory_class="terminator"
                     /note="NOS terminator"
     primer_bind     complement(3860..3876)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3884..3900
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3908..3938)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3953..3974
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."