LOCUS       Exported                4009 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHQEFF-1, complete sequence.
ACCESSION   KM985459
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Ma J-Z., Ma Y-L., Huang F.
  TITLE     Efficient Transient Expression Vectors set for Transactivation 
            Analysis of Plant Transcription Factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Ma J-Z., Ma Y-L., Huang F.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2014) Lanzhou University of Technology, School of 
            Life Science, Qili River Street, Lanzhou, Gansu 730050, China
REFERENCE   3  (bases 1 to 4009)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Cloning vector pHQEFF-1"
                     /mol_type="other DNA"
                     /db_xref="taxon:1608331"
     rep_origin      complement(183..771)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(942..1802)
                     /codon_start=1
                     /product="ampicillin resistance protein"
                     /label=ampicillin resistance protein
                     /protein_id="AJK30597.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1803..1907)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2381..2397
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2414..2944
                     /regulatory_class="promoter"
                     /note="35S; derived from CaMV"
     promoter        2597..2942
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    2971..3408
                     /label=Gal4 DNA binding domain
                     /note="Gal4 DNA binding domain"
     promoter        3418..3436
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3454..3486
                     /label=c-Myc epitope tag
                     /note="c-Myc epitope tag"
     CDS             3456..3485
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      3516..3768
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      3535..3771
                     /regulatory_class="terminator"
                     /note="NOS"
     primer_bind     complement(3790..3806)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3814..3830
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3838..3868)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3883..3904
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."