LOCUS       Exported               12196 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning and transformation vector pHPdestVenus, complete sequence.
ACCESSION   GU142865
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12196)
  AUTHORS   Boy AL, Zhai Z, Habring-Mueller A, Kaspar P, Lohmann I.
  TITLE     Vectors for efficient and high-throughput construction of 
            fluorescent Drosophila reporters using the PhiC31 site-specific 
            integration system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12196)
  AUTHORS   Lohmann I, Boy AL, Habring-Mueller A.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2009) CellNetworks - Cluster of Excellence and 
            BIOQUANT Center, University of Heidelberg, Im Neuenheimer Feld 267, 
            Heidelberg 69120, Germany
REFERENCE   3  (bases 1 to 12196)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12196
                     /organism="Cloning and transformation vector pHPdestVenus"
                     /mol_type="other DNA"
                     /db_xref="taxon:693962"
     protein_bind    63..187
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    188..1642
                     /label=Gateway cassette
                     /note="Gateway cassette"
     promoter        212..242
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             296..955
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1297..1602
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1643..1767)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      1779..1911
                     /regulatory_class="promoter"
                     /note="Hsp70 promotor"
     regulatory      1974..1983
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1980..2729
                     /codon_start=1
                     /product="Venus+Strep tag"
                     /label=Venus+Strep tag
                     /protein_id="ACZ51347.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKGAWSHPQFEK"
     CDS             1980..2696
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             2703..2726
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    2856..2977
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    3007..3291
                     /label=attB site for phiC31 integrase
                     /note="attB site for phiC31 integrase"
     protein_bind    3201..3270
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    3297..3683
                     /label=gypsy insulator
                     /note="gypsy insulator"
     protein_bind    3298..3683
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, and Mod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     gene            4163..7043
                     /gene="mini-white"
                     /label=mini-white
     misc_feature    7821..8406
                     /label=P element 5 end
     misc_feature    7822..8406
                     /label=P element 5 prime end
                     /note="P element 5 prime end"
     misc_feature    8407..8634
                     /label=white linker sequence
                     /note="white linker sequence"
     misc_feature    8635..10663
                     /label=pUC8 plasmid DNA
                     /note="pUC8 plasmid DNA"
     rep_origin      complement(8641..9229)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9400..10260)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10261..10365)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    10664..11166
                     /label=white linker sequence
                     /note="white linker sequence"
     misc_feature    11167..11405
                     /label=P element 3 prime end
                     /note="P element 3 prime end"
     misc_feature    11167..11399
                     /label=P element 3 end
                     /note="P element 3 end"
     misc_feature    11778..12193
                     /label=gypsy insulator
                     /note="gypsy insulator"
     protein_bind    11798..12183
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, and Mod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"