LOCUS       Exported                4748 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHP13, complete sequence.
ACCESSION   DQ297764
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Jakobsen OM, Benichou A, Flickinger MC, Valla S, Ellingsen TE, 
            Brautaset T.
  TITLE     Upregulated transcription of plasmid and chromosomal ribulose 
            monophosphate pathway genes is critical for methanol assimilation 
            rate and methanol tolerance in the methylotrophic bacterium Bacillus
            methanolicus
  JOURNAL   J. Bacteriol. 188 (8), 3063-3072 (2006)
  PUBMED    16585766
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Jakobsen OM, Benichou A, Flickinger MC, Valla S, Ellingsen TE, 
            Brautaset T.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-NOV-2005) Department of Biotechnology, Norwegian 
            University of Science and Technology, Sem Saelands vei 6/8, 
            Trondheim 7491, Norway
REFERENCE   3  (bases 1 to 4748)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Cloning vector pHP13"
                     /mol_type="other DNA"
                     /note="vector first presented in: Haima, P., S. Bron, and 
                     G. Venema. 1987. The Effect of Restriction on Shotgun 
                     Cloning and Plasmid Stability in Bacillus-Subtilis Marburg.
                     Molecular & General Genetics 209:335-342. E. coli/B. 
                     subtilis shuttle vector"
                     /db_xref="taxon:360321"
     regulatory      complement(356..415)
                     /regulatory_class="attenuator"
     rep_origin      565..1153
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1441..1462
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        1477..1507
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1515..1531
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1539..1555
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1551..1868
                     /note="partial lacZ fragment from pUC9; 3-end of coding 
                     region ends in arbitrary stop codon inside chloramphenicol 
                     acetyltransferase"
     CDS             1551..1868
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLAAGRRIPGNSLAVVLQRRDWENPGVTQLNRLAAHPPF
                     ASWRNSEEARTDRPSQQLRSLNGEWRLTGQVSDIRKPTVKSTVGIISYYKSQSLGLSDN
                     S"
     primer_bind     complement(1604..1620)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1829..2479)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /note="derived from pC194"
                     /protein_id="ABC40607.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     CDS             2905..3927
                     /codon_start=1
                     /gene="rep60"
                     /product="replication protein"
                     /label=rep60
                     /note="involved in initiation of plasmid replication; 
                     derived from pTA1060"
                     /protein_id="ABC40605.1"
                     /translation="MIYSSENDYSILEDKTATGKKRDWKGKKRRTNLMAEHYEALESKI
                     GAPYYGKKAEKLISCAEYLSFKRDPETGKLKLYQAHFCKVRLCPMCAWRRSLKIAYHNK
                     LIVEEANRQYGCGWIFLTLTIRNVKGERLKPQISAMMEGFRKLFQYKKVKTSVLGFFRA
                     LEITKNHEEDTYHPHFHVLIPVRKNYFGKNYIKQAEWTSLWKKAMKLDYTPIVDIRRVK
                     GKAKIDAEQIENDVRNAMMEQKAVLEISKYPVKDTDVVRGNKVTEDNLNTVLYLDDALA
                     ARRLIGYGGILKEIHKELNLGDAEDGDLVKIEEEDDEVANGAFEVMAYWHPGIKNYIIK
                     "
     gene            2905..3927
                     /gene="rep60"
                     /label=rep60
     CDS             complement(4310..296)
                     /codon_start=1
                     /product="adenine methylase"
                     /label=adenine methylase
                     /note="derived from pE194; mls resistance"
                     /protein_id="ABC40606.1"
                     /translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFT
                     LELVKRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIYGNIPY
                     NISTDIIRKIVFDSIANEIYLIVEYGFAKRLLNTKRSLALLLMAEVDISILSMVPREYF
                     HPKPKVNSSLIRLSRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGID
                     DLNNISFEQFLSLFNSYKLFNK"